MCD Diet Rat Model Induces Alterations in Zinc and Iron during NAFLD Progression from Steatosis to Steatohepatitis.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
19 Jun 2022
Historique:
received: 30 05 2022
revised: 15 06 2022
accepted: 17 06 2022
entrez: 24 6 2022
pubmed: 25 6 2022
medline: 28 6 2022
Statut: epublish

Résumé

We evaluate the effects of the methionine-choline-deficient (MCD) diet on serum and hepatic zinc (Zn) and iron (Fe) and their relationships with matrix metalloproteinases (MMPs) and their modulators (TIMPs and RECK) as well as hepatic fatty acids using male Wistar rats fed 2-, 4- and 8-week MCD diets. Serum and hepatic Zn decrease after an 8-week MCD diet. Serum Fe increases after an 8-week MCD diet and the same occurs for hepatic Fe. An increase in hepatic MMP activity, associated with a decrease in RECK and TIMPs, is found in the MCD 8-week group. Liver Fe shows a positive correlation versus MMPs and RECK, and an inverse correlation versus TIMPs. A positive correlation is found comparing liver Zn with stearic, vaccenic and arachidonic acids, and an inverse correlation is found with linolenic and docosatetraenoic acids. An opposite trend is found between liver Fe versus these fatty acids. During NAFLD progression from steatosis to steatohepatitis, MCD rats exhibit an increase in Zn and a decrease in Fe levels both in serum and tissue associated with alterations in hepatic MMPs and their inhibitors, and fatty acids. The correlations detected between Zn and Fe versus extracellular matrix modulators and fatty acids support their potential role as therapeutic targets.

Identifiants

pubmed: 35743260
pii: ijms23126817
doi: 10.3390/ijms23126817
pmc: PMC9224179
pii:
doi:

Substances chimiques

Fatty Acids 0
Methionine AE28F7PNPL
Iron E1UOL152H7
Zinc J41CSQ7QDS
Choline N91BDP6H0X

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Giuseppina Palladini (G)

Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy.
Fondazione IRCCS (Istituti di Ricovero e Cura a Carattere Scientifico) Policlinico San Matteo, 27100 Pavia, Italy.

Laura Giuseppina Di Pasqua (LG)

Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy.

Marta Cagna (M)

Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy.

Anna Cleta Croce (AC)

Institute of Molecular Genetics, Italian National Research Council (CNR), 27100 Pavia, Italy.

Stefano Perlini (S)

Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy.
Emergency Department, Fondazione IRCCS (Istituti di Ricovero e Cura a Carattere Scientifico) Policlinico San Matteo, 27100 Pavia, Italy.

Barbara Mannucci (B)

Centro Grandi Strumenti, University of Pavia, 27100 Pavia, Italy.

Antonella Profumo (A)

Department of Chemistry, University of Pavia, 27100 Pavia, Italy.

Andrea Ferrigno (A)

Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy.

Mariapia Vairetti (M)

Department of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, Italy.

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Classifications MeSH