High Throughput Screening of Antimicrobial Resistance Genes in Gram-Negative Seafood Bacteria.
Gram-negative species
antimicrobial resistance genes
high throughput qPCR
micro-array
mobile genetic elements
seafood bacteria
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
15 Jun 2022
15 Jun 2022
Historique:
received:
28
03
2022
revised:
10
06
2022
accepted:
11
06
2022
entrez:
24
6
2022
pubmed:
25
6
2022
medline:
25
6
2022
Statut:
epublish
Résumé
From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This study aims to provide new data and insights regarding the content of resistance markers in various seafood samples and sources, and therefore the potential exposure to humans in a global One Health approach. An innovative high throughput qPCR screening was developed and validated in order to simultaneously investigate the presence of 41 ARGs and 33 MGEs including plasmid replicons, integrons, and insertion sequences in Gram-negative bacteria. Analysis of 268 seafood isolates from the bacterial microflora of cod (
Identifiants
pubmed: 35744743
pii: microorganisms10061225
doi: 10.3390/microorganisms10061225
pmc: PMC9230514
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : French research government and the "Hauts de France" Region
ID : CPER 2014-2020 MARCO
Organisme : French General Directorate for Food
ID : "EcoAntibio2", Program, 2017
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