Novel High-Throughput Fluorescence-Based Assay for the Identification of Nematocidal Compounds That Target the Blood-Feeding Pathway.

blood-feeding drug-screening fluorescence helminth hookworm motility viability

Journal

Pharmaceuticals (Basel, Switzerland)
ISSN: 1424-8247
Titre abrégé: Pharmaceuticals (Basel)
Pays: Switzerland
ID NLM: 101238453

Informations de publication

Date de publication:
27 May 2022
Historique:
received: 26 04 2022
revised: 23 05 2022
accepted: 24 05 2022
entrez: 24 6 2022
pubmed: 25 6 2022
medline: 25 6 2022
Statut: epublish

Résumé

Hookworm infections cause a neglected tropical disease (NTD) affecting ~740 million people worldwide, principally those living in disadvantaged communities. Infections can cause high morbidity due to their impact on nutrient uptake and their need to feed on host blood, resulting in a loss of iron and protein, which can lead to severe anaemia and impaired cognitive development in children. Currently, only one drug, albendazole is efficient to treat hookworm infection and the scientific community fears the rise of resistant strains. As part of on-going efforts to control hookworm infections and its associated morbidities, new drugs are urgently needed. We focused on targeting the blood-feeding pathway, which is essential to the parasite survival and reproduction, using the laboratory hookworm model

Identifiants

pubmed: 35745589
pii: ph15060669
doi: 10.3390/ph15060669
pmc: PMC9231213
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Swiss National Science Foundation
ID : PR00P3_193084
Pays : Switzerland
Organisme : European Society of Clinical Microbiology and Infectious Diseases
ID : Research Grant 2017
Organisme : Swiss National Science Foundation
ID : national project NCCR-Chemical Biology
Pays : Switzerland
Organisme : National Health and Medical Research Council
ID : National Health and Medical Research Council (NHMRC) SRF-B
Organisme : Australian Research Council
ID : LP180101085
Organisme : Australian Research Council
ID : LP190101209
Organisme : Australian Research Council
ID : DP210101500

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Auteurs

Anthony Marchand (A)

Laboratory of Intestinal Immunology, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

Joyce W M Van Bree (JWM)

Department of Immunology and Pathology, Alfred Medical Research and Education Precinct (AMREP), Monash University, Melbourne, VIC 3004, Australia.

Aya C Taki (AC)

Melbourne Veterinary School, The University of Melbourne, Melbourne, VIC 3052, Australia.

Mati Moyat (M)

Laboratory of Intestinal Immunology, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Department of Immunology and Pathology, Alfred Medical Research and Education Precinct (AMREP), Monash University, Melbourne, VIC 3004, Australia.

Gerardo Turcatti (G)

Biomolecular Screening Facility, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

Marc Chambon (M)

Biomolecular Screening Facility, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

Adam Alexander Thil Smith (AAT)

Metabolomics Laboratory, Baker Heart and Diabetes Institute, Melbourne, VIC 3004, Australia.

Rory Doolan (R)

Hookworm Immuno-Biology Laboratory, Swiss Tropical and Public Health Institute, 4123 Allschwill, Switzerland.
Basel University, 4001 Basel, Switzerland.

Robin B Gasser (RB)

Melbourne Veterinary School, The University of Melbourne, Melbourne, VIC 3052, Australia.

Nicola Laraine Harris (NL)

Laboratory of Intestinal Immunology, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Department of Immunology and Pathology, Alfred Medical Research and Education Precinct (AMREP), Monash University, Melbourne, VIC 3004, Australia.

Tiffany Bouchery (T)

Laboratory of Intestinal Immunology, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Department of Immunology and Pathology, Alfred Medical Research and Education Precinct (AMREP), Monash University, Melbourne, VIC 3004, Australia.
Hookworm Immuno-Biology Laboratory, Swiss Tropical and Public Health Institute, 4123 Allschwill, Switzerland.
Basel University, 4001 Basel, Switzerland.

Classifications MeSH