X-ray Stain Localization with Near-Field Ptychographic Computed Tomography.
contrast agents
ptychographic computed tomography
quantitative X-ray imaging
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
17
05
2022
received:
24
03
2022
pubmed:
25
6
2022
medline:
27
8
2022
entrez:
24
6
2022
Statut:
ppublish
Résumé
Although X-ray contrast agents offer specific characteristics in terms of targeting and attenuation, their accumulation in the tissue on a cellular level is usually not known and difficult to access, as it requires high resolution and sensitivity. Here, quantitative near-field ptychographic X-ray computed tomography is demonstrated to assess the location of X-ray stains at a resolution sufficient to identify intracellular structures by means of a basis material decomposition. On the example of two different X-ray stains, the nonspecific iodine potassium iodide, and eosin Y, which mostly interacts with proteins and peptides in the cell cytoplasm, the distribution of the stains within the cells in murine kidney samples is assessed and compared to unstained samples with similar structural features. Quantitative nanoscopic stain concentrations are in good agreement with dual-energy micro computed tomography measurements, the state-of-the-art modality for material-selective imaging. The presented approach can be applied to a variety of X-ray stains advancing the development of X-ray contrast agents.
Identifiants
pubmed: 35748171
doi: 10.1002/advs.202201723
pmc: PMC9404393
doi:
Substances chimiques
Coloring Agents
0
Contrast Media
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2201723Subventions
Organisme : Deutsche Forschungsgemeinschaft: Research Training Group
ID : GRK 2274
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
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