Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
21
03
2022
accepted:
17
05
2022
entrez:
24
6
2022
pubmed:
25
6
2022
medline:
29
6
2022
Statut:
epublish
Résumé
Leishmaniasis is a parasitic vector-borne disease caused by the protistan flagellates of the genus Leishmania. Leishmania (Viannia) guyanensis is one of the most common causative agents of the American tegumentary leishmaniasis. It has previously been shown that L. guyanensis strains that carry the endosymbiotic Leishmania RNA virus 1 (LRV1) cause more severe form of the disease in a mouse model than those that do not. The presence of the virus was implicated into the parasite's replication and spreading. In this respect, studying the molecular mechanisms of cellular control of viral infection is of great medical importance. Here, we report ~30.5 Mb high-quality genome assembly of the LRV1-positive L. guyanensis M4147. This strain was turned into a model by establishing the CRISPR-Cas9 system and ablating the gene encoding phosphatidate phosphatase 2-like (PAP2L) protein. The orthologue of this gene is conspicuously absent from the genome of an unusual member of the family Trypanosomatidae, Vickermania ingenoplastis, a species with mostly bi-flagellated cells. Our analysis of the PAP2L-null L. guyanensis showed an increase in the number of cells strikingly resembling the bi-flagellated V. ingenoplastis, likely as a result of the disruption of the cell cycle, significant accumulation of phosphatidic acid, and increased virulence compared to the wild type cells.
Identifiants
pubmed: 35749562
doi: 10.1371/journal.pntd.0010510
pii: PNTD-D-22-00362
pmc: PMC9232130
doi:
Substances chimiques
Lipids
0
Phosphatidate Phosphatase
EC 3.1.3.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0010510Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Infect Genet Evol. 2017 Jun;50:121-127
pubmed: 27381333
J Infect Dis. 2016 Jan 1;213(1):105-11
pubmed: 26123564
Parasitology. 2021 Sep;148(10):1161-1170
pubmed: 33407966
PLoS One. 2018 Feb 13;13(2):e0192723
pubmed: 29438445
Bioinformatics. 2018 Jul 1;34(13):i142-i150
pubmed: 29949969
Prog Lipid Res. 2006 May;45(3):250-78
pubmed: 16574237
Nat Rev Microbiol. 2007 Apr;5(4):287-97
pubmed: 17363967
J Vector Ecol. 2011 Mar;36 Suppl 1:S1-9
pubmed: 21366760
Trans R Soc Trop Med Hyg. 1979;73(2):239-42
pubmed: 473314
PLoS Negl Trop Dis. 2016 Mar 03;10(3):e0004349
pubmed: 26937644
PLoS One. 2017 Oct 26;12(10):e0185056
pubmed: 29073143
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5979-82
pubmed: 2762308
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9572-5
pubmed: 3200841
J Clin Invest. 2008 Apr;118(4):1301-10
pubmed: 18382742
Biol Rev Camb Philos Soc. 2022 Feb;97(1):141-162
pubmed: 34472688
Nat Commun. 2019 Nov 21;10(1):5273
pubmed: 31754185
Gigascience. 2012 Dec 27;1(1):18
pubmed: 23587118
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Nat Biotechnol. 2011 May 15;29(7):644-52
pubmed: 21572440
Mol Biochem Parasitol. 1991 Apr;45(2):307-15
pubmed: 1903845
FEBS Lett. 2014 Mar 3;588(5):783-8
pubmed: 24492004
PLoS One. 2013 Nov 21;8(11):e81397
pubmed: 24278433
Mol Biol Evol. 2012 Oct;29(10):2921-36
pubmed: 22491036
Sci Rep. 2016 Mar 29;6:23704
pubmed: 27021793
Biochem J. 1999 Jun 15;340 ( Pt 3):677-86
pubmed: 10359651
Pathog Dis. 2018 Jun 1;76(4):
pubmed: 29722820
Virulence. 2021 Dec;12(1):852-867
pubmed: 33724149
Genome Res. 2010 Sep;20(9):1297-303
pubmed: 20644199
J Biol Chem. 2006 Apr 7;281(14):9297-306
pubmed: 16467304
Nucleic Acids Res. 2010 Jan;38(Database issue):D457-62
pubmed: 19843604
Trans R Soc Trop Med Hyg. 2011 Oct;105(10):579-85
pubmed: 21890159
Bull Soc Pathol Exot Filiales. 1954;47(6):784-7
pubmed: 14378914
Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):11998-12005
pubmed: 27790981
Pathogens. 2021 Sep 02;10(9):
pubmed: 34578156
Lipids. 2011 Oct;46(10):969-79
pubmed: 21667213
J Eukaryot Microbiol. 2016 Mar-Apr;63(2):198-209
pubmed: 26352484
PLoS Negl Trop Dis. 2013;7(1):e2006
pubmed: 23326619
J Infect Dis. 2016 Jan 1;213(1):112-21
pubmed: 26123565
Nat Genet. 2007 Jul;39(7):839-47
pubmed: 17572675
Nat Genet. 2014 Aug;46(8):912-918
pubmed: 25017105
PLoS Pathog. 2019 Jun 26;15(6):e1007828
pubmed: 31242261
Parasit Vectors. 2012 Dec 03;5:276
pubmed: 23206339
Biochim Biophys Acta. 2003 Nov 30;1635(1):1-9
pubmed: 14642771
Bioinformatics. 2018 Sep 1;34(17):i884-i890
pubmed: 30423086
Sci Rep. 2020 Aug 6;10(1):13209
pubmed: 32764655
J Biol Chem. 2012 May 18;287(21):17186-17197
pubmed: 22474284
J Biol Chem. 1957 May;226(1):497-509
pubmed: 13428781
Proc Natl Acad Sci U S A. 2021 Oct 19;118(42):
pubmed: 34654744
Genome Res. 2006 Sep;16(9):1159-68
pubmed: 16951135
Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E811-E819
pubmed: 28096399
Biochem J. 2005 Apr 15;387(Pt 2):281-93
pubmed: 15801912
Microbiol Resour Announc. 2018 Dec 13;7(23):
pubmed: 30574587
J Eukaryot Microbiol. 2006 Mar-Apr;53(2):103-11
pubmed: 16579812
Infect Genet Evol. 2016 Mar;38:101-109
pubmed: 26708057
Trends Biochem Sci. 2006 Dec;31(12):694-9
pubmed: 17079146
Proc Natl Acad Sci U S A. 2017 May 9;114(19):4987-4992
pubmed: 28439019
Trends Parasitol. 2018 Jun;34(6):466-480
pubmed: 29605546
Mem Inst Oswaldo Cruz. 2009 Jul;104 Suppl 1:311-8
pubmed: 19753490
J Eukaryot Microbiol. 2022 Feb 17;:e12897
pubmed: 35175680
R Soc Open Sci. 2018 Apr 25;5(4):172212
pubmed: 29765675
Nucleic Acids Res. 2016 Jul 8;44(W1):W29-34
pubmed: 27105845
Sci Rep. 2015 Feb 24;5:8550
pubmed: 25707621
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3036-41
pubmed: 9501211
PLoS Pathog. 2010 Oct 28;6(10):e1001161
pubmed: 21060810
Cell Death Discov. 2019 Jun 24;5:107
pubmed: 31263575
FEBS Lett. 1977 Aug 15;80(2):360-4
pubmed: 142663
mBio. 2015 Sep 15;6(5):e01356-15
pubmed: 26374124
J Biol Chem. 2012 Nov 23;287(48):40186-96
pubmed: 23071111
J Biol Chem. 2002 Nov 22;277(47):45226-34
pubmed: 12198133
PLoS Negl Trop Dis. 2014 Apr 24;8(4):e2836
pubmed: 24762979
Methods Mol Biol. 2019;1962:227-245
pubmed: 31020564
Mol Biochem Parasitol. 2015 Mar-Apr;200(1-2):30-40
pubmed: 25736427
Curr Genet. 2008 May;53(5):313-22
pubmed: 18343926
J Biol Chem. 1998 Aug 21;273(34):22059-67
pubmed: 9705349
Cell Host Microbe. 2016 Mar 9;19(3):349-60
pubmed: 26962945
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
J Biol Chem. 1985 Dec 15;260(29):15646-54
pubmed: 2999127
PLoS Pathog. 2021 Jun 11;17(6):e1009654
pubmed: 34115806
Viruses. 2020 Feb 02;12(2):
pubmed: 32024293
Trends Parasitol. 2021 Feb;37(2):100-116
pubmed: 33127331
Bioinformatics. 2015 Aug 1;31(15):2443-51
pubmed: 25810435
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
Pathogens. 2021 Jan 14;10(1):
pubmed: 33466586
Curr Protoc Bioinformatics. 2002 Aug;Chapter 2:Unit 2.3
pubmed: 18792934
Bioeng Bugs. 2011 Nov-Dec;2(6):320-6
pubmed: 22067831
J Biol Chem. 1991 Feb 15;266(5):2988-96
pubmed: 1993672
Bioinformatics. 2008 Oct 15;24(20):2317-23
pubmed: 18718941
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
Methods Mol Biol. 2019;1971:189-210
pubmed: 30980304
J Mol Biol. 2001 Jan 19;305(3):567-80
pubmed: 11152613
Mol Biochem Parasitol. 2014 Jul;195(2):115-22
pubmed: 24893339
Bioinformatics. 2009 Aug 1;25(15):1972-3
pubmed: 19505945
Cell Microbiol. 2015 Oct;17(10):1405-12
pubmed: 25939677
Chem Rev. 2012 Oct 10;112(10):5121-46
pubmed: 22742522
Mol Biochem Parasitol. 2006 Jun;147(2):193-206
pubmed: 16546274
Trends Parasitol. 2006 Sep;22(9):439-45
pubmed: 16843727
Trans R Soc Trop Med Hyg. 1981;75(4):611-2
pubmed: 7324143
J Eukaryot Microbiol. 2016 Sep;63(5):657-78
pubmed: 27009761
Eukaryot Cell. 2011 Nov;10(11):1429-38
pubmed: 21926331
PLoS Negl Trop Dis. 2017 Jul 24;11(7):e0005782
pubmed: 28742133
Mol Biol Evol. 2011 Jan;28(1):343-9
pubmed: 20671039
Mol Biochem Parasitol. 2014 Nov;198(1):11-3
pubmed: 25461484
Nat Protoc. 2013 Aug;8(8):1494-512
pubmed: 23845962
Trends Parasitol. 2020 Sep;36(9):745-760
pubmed: 32703742
mBio. 2021 Aug 31;12(4):e0160621
pubmed: 34399629
Adv Parasitol. 1999;42:29-112
pubmed: 10050272
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
PLoS Pathog. 2015 Aug 28;11(8):e1005127
pubmed: 26317207
Mol Microbiol. 2011 Feb;79(3):647-62
pubmed: 21255109
Science. 2011 Feb 11;331(6018):775-8
pubmed: 21311023
J Biol Chem. 1999 May 21;274(21):14831-7
pubmed: 10329682
Parasitology. 2019 Jan;146(1):1-27
pubmed: 29898792
Open Biol. 2021 Mar;11(3):200407
pubmed: 33715388
PLoS Negl Trop Dis. 2021 Dec 29;15(12):e0010041
pubmed: 34965251
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
BMC Genomics. 2019 Apr 18;19(Suppl 9):238
pubmed: 30999844
J Biol Chem. 1996 Jul 12;271(28):16506-9
pubmed: 8663293
BMC Biol. 2020 Dec 2;18(1):187
pubmed: 33267865
Microb Cell Fact. 2013 Jan 29;12:9
pubmed: 23356794
Trop Med Infect Dis. 2020 Feb 13;5(1):
pubmed: 32069939
R Soc Open Sci. 2017 May 3;4(5):170095
pubmed: 28573017