Effectiveness of using liquid transport media in bacteriological diagnostics of diphtheria infection.
Эффективность использования жидких транспортных сред в бактериологической диагностике дифтерийной инфекции.
Amies liquid transport media
Corynebacterium diphtheriae
bacteriological diagnosis
biological material
storage conditions
transport media
Journal
Klinicheskaia laboratornaia diagnostika
ISSN: 0869-2084
Titre abrégé: Klin Lab Diagn
Pays: Russia (Federation)
ID NLM: 9432021
Informations de publication
Date de publication:
20 Jun 2022
20 Jun 2022
Historique:
entrez:
24
6
2022
pubmed:
25
6
2022
medline:
29
6
2022
Statut:
ppublish
Résumé
The results of evaluating the effectiveness of the use of liquid transport media at the preanalytical stage of bacteriological diagnosis of diphtheria infection are presented. A typical toxigenic strain of C. diphtheriae biovar gravis № 665 was used. The experiments were carried out using a laboratory-prepared medium based on GRM-broth (State research center for applied biotechnology and microbiology, Obolensk), a transport system with a fleecy probe swab (DELTALAB) and a transport system ∑-Transwab ® with a polyurethane Sigma-swab (Medical Wire & Equipment Co. (Bath) Ltd.). The tampons were pooled with a 24-hour bacterial culture of C. diphtheriae, then immediately seeded on Tellurite-containing blood agar. Storage conditions were simulated for 6-24 hours: at room conditions +(20-25)° C, in the refrigerator +(4-8)° C, in a thermostat +(37±1)° C. Storage of C. diphtheriae was most optimal on two liquid transport systems in a refrigerator +(4-8)° C for 6 and 24 hours; in room conditions +(20-25)° C - there was a decrease in seeding after 6 hours and loss of pathological material after 24 hours, more pronounced on a fleecy probe swab; under thermostat conditions +(37±1)° C on both transport systems, a decrease in seeding was noted after 6 hours and a complete loss of pathological material after 24 hours. The results obtained demonstrated the efficiency of using the Amies liquid transport medium and justify the need to develop a domestic analogue of the transport system based on the Amies liquid medium for the bacteriological diagnosis of diphtheria infection.
Identifiants
pubmed: 35749600
doi: 10.51620/0869-2084-2022-67-6-350-354
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
350-354Subventions
Organisme : The Rospotrebnadzor
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Methods of clinical laboratory research. Vol. 3. Menshikov V.V., ed. [Metodiki klinicheskikh laboratornykh issledovaniy. Tom 3. Men’shikov V.V., ed.]. Moscow: Labora; 2009. (in Russian)
Avolio M., Camporese A. Liqiud based microbiological transport systems: conformity assessment of two commercial devices. J. Microbiol methods. 2015; 115: 42-4.
Arbique J.C., Forward K.R., LeBlanc J. Evaluation of four commercial transport media for the survival of Neisseria gonorrhoeae. Diagn. Microbiol. Infect. Dis. 2000; 36(3): 163-8.
Buchan B.W., Olson W.J., Mackey T.L., Ledeboer N.A. Clinical evaluation of the walk-away specimen processor and ESwab for recovery of Streptococcus agalactiae isolates in prenatal screening specimens. J. Clin. Microbiol. 2014; 52(6): 2166-8.
Hirvonen J.J., Kaukoranta S.S. Comparison of FecalSwab and ESwab devices for storage and transportation of diarrheagenic bacteria. J. Clin. Microbiol. 2014; 52(7): 2334-9.
Nys S., Vijgen S., Magerman K., Cartuyvels R. Comparison of Copan eSwab with the Copan Venturi Transystem for the quantitative survival of Escherichia coli, Streptococcus agalactiae and Candida albicans. Eur. J. Clin. Microbiol. Infect. Dis. 2010; 29(4): 453-6.
Van Horn K.G., Audette C.D., Tucker K.A., Sebeck D. Comparison of 3 swab transport systems for direct release and recovery of aerobic and anaerobic bacteria. Diagn. Microbiol. Infect. Dis. 2008; 62(4): 471-3.
Chagina I.A., Borisova O. Yu., Gadua N.T., Afanasiev M.S., Afanasiev S.S., Skvortsov A.G. Possibilities of using a liquid transport media to take pathological material in laboratory diagnostics of diphtheria infection. Klinicheskaya Laboratornaya Diagnostika. 2018; 63(12): 783-7. (in Russian)
On the results of external quality control of studies on bacteriological diagnostics of diphtheria and pertussis and serological diagnostics of diphtheria in Russian Federation in 2019. Information letter of Rospotrebnadzor dated 03.09.2019. No. 02/12550-2019-32. [O rezul’tatah provedeniya vneshnego kontrolya kachestva issledovanij po bakteriologicheskoj diagnostike difterii i koklyusha i serologicheskoj diagnostike difterii v Rossijskoj Federacii v 2019 godu. Informacionnoe pis’mo Rospotrebnadzora ot 03.09.2019 g. № 02/12550-2019-32.] (in Russian)
On the results of external quality control of studies on bacteriological diagnostics of diphtheria and pertussis and serological diagnostics of diphtheria in Russian Federation in 2020. Information letter of Rospotrebnadzor dated 16.11.2020. No. 02/23440-2020-27. [O rezul’tatah provedeniya vneshnego kontrolya kachestva issledovanij po bakteriologicheskoj diagnostike difterii i koklyusha i serologicheskoj diagnostike difterii v Rossijskoj Federacii v 2020 godu. Informacionnoe pis’mo Rospotrebnadzora ot 16.11.2020 g. № 02/23440-2020-27.] (in Russian)
On the results of external quality control of studies on bacteriological diagnostics of diphtheria and pertussis and serological diagnostics of diphtheria in Russian Federation in 2021. Information letter of Rospotrebnadzor dated 12.10.2021. No. 02/20615-2021-27. [O rezul’tatah provedeniya vneshnego kontrolya kachestva issledovanij po bakteriologicheskoj diagnostike difterii i koklyusha i serologicheskoj diagnostike difterii v Rossijskoj Federacii v 2021 godu. Informacionnoe pis’mo Rospotrebnadzora ot 12.10.2021 g. № 02/20615-2021-27.] (in Russian)
Borisova O.Yu., Gadua N.T., Pimenova A.S., Afanasiev S.S., Afanasiev M.S., Mironov A. Yu. et al. State and problems of bacteriological diagnosis of diphtheria infection in Russian Federation. Klinicheskaya Laboratornaya Diagnostika. 2020; 65(11): 717-23. (in Russian)
Borisova O.Yu., Chagina I.A., Gadua N.T. On the use of commercial transport media for taking pathological material when examining for diphtheria. Meditsinskiy alfavit. Sovremennaya laboratoriya. 2014; 3: 21-3. (in Russian)
Shepelin A.P., Domotenko L.V., Dyatlov I.A., Mironov A.Yu., Aleshkin V.A. The actual approaches to problem of import substitution in th field of production growth medium. Klinicheskaya Laboratornaya Diagnostika. 2015; 60(6): 63-5. (in Russian)
Dyatlov I.A., Mironov A.Yu., Shepelin A.P., Aleshkin V.A. The condition and tendencies of development of clinical and sanitary microbiology in the russian federation and problem of import substitution. Klinicheskaya Laboratornaya Diagnostika. 2015; 60(8): 61-5. (in Russian)