Downregulation of CDC42 inhibits the proliferation and stemness of human trophoblast stem cell via EZRIN/YAP inactivation.

Cell Proliferation Cytoskeletal Proteins / metabolism Down-Regulation Female Humans Placenta / metabolism Pregnancy Stem Cells Trophoblasts / cytology YAP-Signaling Proteins / metabolism cdc42 GTP-Binding Protein / metabolism
CDC42 EZRIN Human trophoblast stem cell Placenta Recurrent spontaneous abortion YAP

Journal

Cell and tissue research
ISSN: 1432-0878
Titre abrégé: Cell Tissue Res
Pays: Germany
ID NLM: 0417625

Informations de publication

Date de publication:
Sep 2022
Historique:
received: 20 10 2021
accepted: 09 06 2022
pubmed: 26 6 2022
medline: 8 9 2022
entrez: 25 6 2022
Statut: ppublish

Résumé

Placental dysplasia increases the risk of recurrent spontaneous abortion (RSA). However, the underlying mechanism regulating placental development remains unclear. In this study, we showed that the expression of CDC42 was decreased in the villous tissue of RSA samples compared to healthy controls. Further examination demonstrated that CDC42 deficiency led to the differentiation of human trophoblast stem cells (hTSCs) and inhibited their proliferation. Genetic manipulation of YAP and EZRIN in hTSCs revealed that CDC42 regulates the stemness and proliferation of hTSCs; this is dependent on EZRIN, which translocates YAP into the nucleus. Moreover, the expression pattern of EZRIN, YAP, and Ki67 was also abnormal in the villous tissue of RSA samples, consistent with in vitro experiments. In summary, these findings suggest that the CDC42/EZRIN/YAP pathway plays an important role in placental development.

Identifiants

DOI: 10.1007/s00441-022-03653-6 PMID: 35751703
pubmed: 35751703
doi: 10.1007/s00441-022-03653-6
pii: 10.1007/s00441-022-03653-6
doi:

Substances chimiques

Cytoskeletal Proteins 0
YAP-Signaling Proteins 0
YAP1 protein, human 0
ezrin 0
CDC42 protein, human EC 3.6.5.2
cdc42 GTP-Binding Protein EC 3.6.5.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

573-585

Subventions

Organisme : National Natural Science Foundation of China
ID : 81830045
Organisme : National Natural Science Foundation of China
ID : 82071652
Organisme : National Natural Science Foundation of China
ID : 31971071
Organisme : National Key R&D Program of China
ID : 2017YFC1001402
Organisme : National Key R&D Program of China
ID : 2018YFC1004102
Organisme : Science and Technology Projects in Guangzhou
ID : 202102010005
Organisme : Guangzhou Municipal Health Commission
ID : 2019GX03
Organisme : General Program of Guangdong Province Natural Science Foundation
ID : 2021A1515011039
Organisme : General Program of Guangdong Province Natural Science Foundation
ID : 2020A1515010273
Organisme : National Natural Science Foundation of China
ID : 81801446

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Bi Shilei (B)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Zhang Lizi (Z)

Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Huang Lijun (H)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Ma Weixu (M)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Meng Nan (M)

Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, China.

Deng Weinan (D)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Li Yulian (L)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Liang Yingyu (L)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Huang Minshan (H)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Xu Pei (X)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Liu Mingxing (L)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Chen Jingsi (C)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Tu Zhaowei (T)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China.

Wang Zhijian (W)

Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Wang Haibin (W)

Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, China.

Lu Jinhua (L)

Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, China. jinhua888@126.com.

Chen Dunjin (C)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China. gzdrchen@gzhmu.edu.cn.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China. gzdrchen@gzhmu.edu.cn.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China. gzdrchen@gzhmu.edu.cn.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China. gzdrchen@gzhmu.edu.cn.

Du Lili (D)

Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou, China. lilidugysy@gzhmu.edu.cn.
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, China. lilidugysy@gzhmu.edu.cn.
Guangdong Engineering and Technology Research Center of Maternal-Fetal Medicine, Guangzhou, China. lilidugysy@gzhmu.edu.cn.
Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, China. lilidugysy@gzhmu.edu.cn.
ORCID: 0000-0003-4014-6138

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Régulation négative › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Structures de l'embryon › Placenta › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Cellules › Cellules souches › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Structures de l'embryon › Placenta › Trophoblastes › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Protéines de signalisation YAP › Downregulation of CDC42 inhibits the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › Protéines G monomériques › Protéines G rho › Protéine G cdc42 › Downregulation of CDC42 inhibits the...