Biological relevance of trophectoderm morphology: initial β-hCG measurements correlate with trophectoderm grading on euploid frozen embryo transfers.
Blastocyst
Embryo implantation
Human chorionic gonadotropin
In vitro fertilization
Pregnancy test
Trophectoderm
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
07
04
2022
accepted:
07
06
2022
pubmed:
26
6
2022
medline:
17
9
2022
entrez:
25
6
2022
Statut:
ppublish
Résumé
To analyze the correlation between TE grading and initial β-hCG serum level after single euploid embryo transfer. Secondarily, to explore the association between TE grading with subsequent IVF outcomes. Retrospective cohort analysis. Single, academic, private infertility and assisted reproductive care institute. Infertility patients who underwent a single euploid embryo transfer that resulted in a positive pregnancy test. β-hCG measurements. Correlation between TE grade with first β-hCG measurement. Second outcome measurements included ongoing pregnancy, biochemical pregnancy loss, and clinical pregnancy loss rates. 2,798 cases were analyzed. A significant difference in initial β-hCG measurement among groups (TE A: median 143.4 mIU/mL IQR 79.2-211.2; TE B: 119 mIU/mL IQR 57.1-177.8; TE C: 82.4 mIU/mL IQR 36.3-136.4, p ≤ 0.0001) was observed. There was a significant correlation found between the TE grade and β-hCG measurements (p ≤ 0.0001, r In patients with euploid embryos, TE grade correlates with the first pregnancy test measurement of β-hCG. We propose this finding helps to appoint a relevant link between morphology assessment and early embryo development in vivo.
Identifiants
pubmed: 35751829
doi: 10.1007/s10815-022-02553-6
pii: 10.1007/s10815-022-02553-6
pmc: PMC9475011
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2051-2059Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Liu HC, Jones GS, Jones HW Jr, Rozenwaks Z. Mechanisms and factors of early pregnancy wastage in in-vitro fertilization embryo transfer patients. Fertil Steril. 1988;50:95–101.
doi: 10.1016/S0015-0282(16)60015-8
Sung N, Kwak-Kim J, Koo HS, Yang KM. Serum hCG-b levels of postovulatory day 12 and 14 with the sequential application of hCG-b fold change significantly increased predictability of pregnancy outcome after IVF-ET cycle. J Assist Reprod Genet. 2016;33:1185–94.
doi: 10.1007/s10815-016-0744-y
Lambers MJ, van Weering HGI, van’t Grunewold MS, et al. Optimizing hCG cutoff values: a single determination on day 14 or 15 is sufficient for a reliable prediction of pregnancy outcome. Eur J Obstet Gynecol Reprod Biol. 2006;127:94–8.
doi: 10.1016/j.ejogrb.2005.12.023
Chung K, Sammel MD, Coutifaris C, et al. Defining the rise of serum HCG in viable pregnancies achieved through use of IVF. Hum Reprod. 2006;21:823–8.
doi: 10.1093/humrep/dei389
De Neubourg D, Gerris J, Mangelschots K, Van Royen E, Vercruyssen M, Elseviers M. Single top quality embryo transfer as a model for prediction of early pregnancy outcome. Hum Reprod. 2004;19(6):1476–9.
doi: 10.1093/humrep/deh283
Girard JM, Simorre M, Leperlier F, Reignier A, Lefebvre T, Barrière P, Fréour T. Association between early βhCG kinetics, blastocyst morphology and pregnancy outcome in a single-blastocyst transfer program. Eur J Obstet Gynecol Reprod Biol. 2018;225:189–93. https://doi.org/10.1016/j.ejogrb.2018.04.037 .
doi: 10.1016/j.ejogrb.2018.04.037
pubmed: 29734084
Licht P, Russu V, Lehmeyer S, Wildt L. Molecular aspects of direct LH/hCG effects on human endometrium—lessons from intrauterine microdialysis in the human female in vivo. Reprod Biol. 2001;1:10–9.
pubmed: 14666171
Tsampalas M, Gridelet V, Berndt S, Foidart JM, Geenen V, Perrier DS. Human chorionic gonadotropin: a hormone with immunological and angiogenic properties. J Reprod Immunol. 2010;85:93–8.
doi: 10.1016/j.jri.2009.11.008
Richter KS, Harris DC, Daneshmand ST, Shapiro BS. Quantitative grading of a human blastocyst: optimal inner cell mass size and shape. Fertil Steril. 2001;76:1157–67.
doi: 10.1016/S0015-0282(01)02870-9
Kovacic B, Vlaisavljevic V, Reljic M, Cizek-Sajko M. Developmental capacity of different morphological types of day 5 human morulae and blastocysts. Reprod Biomed Online. 2004;8:687–94.
doi: 10.1016/S1472-6483(10)61650-1
Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Thomas S. Large blastocyst diameter, early blastulation, and low preovulatory serum progesterone are dominant predictors of clinical pregnancy in fresh autologous cycles. Fertil Steril. 2008;90:302–9.
doi: 10.1016/j.fertnstert.2007.06.062
Van den Abbeel E, Balaban B, Ziebe S, et al. Association between blastocyst morphology and outcome of single-blastocyst transfer. Reprod Biomed Online. 2013;27:353–61.
doi: 10.1016/j.rbmo.2013.07.006
Nazem TG, Sekhon L, Lee JA, Overbey J, Pan S, Duke M, Briton-Jones C, Whitehouse M, Copperman AB, Stein DE. The correlation between morphology and implantation of euploid human blastocysts. Reprod Biomed Online. 2019;38(2):169–76. https://doi.org/10.1016/j.rbmo.2018.10.007 .
doi: 10.1016/j.rbmo.2018.10.007
pubmed: 30579820
Ahlström A, Westin C, Reismer E, Wikland M, Hardarson T. Trophectoderm morphology: an important parameter for predicting live birth after single blastocyst transfer. Hum Reprod. 2011;26:3289–96.
doi: 10.1093/humrep/der325
Zaninovic N, Berrios R, Clarke RN, Bodine R, Ye Z, Veeck LL. Blastocyst expansion, inner cell mass (ICM) formation, and trophectoderm (TM) quality: is one more important for implantation? Fertil Steril. 2001;76:S8.
doi: 10.1016/S0015-0282(01)02038-6
Dokras A, Sargent IL, Barlow DH. Human blastocyst grading: an indicator of developmental potential? Hum Reprod. 1993;8:2119–27.
doi: 10.1093/oxfordjournals.humrep.a137993
Oron G, Shavit T, Esh-Broder E, Weon-Young S, Tulandi T, Holzer H. Predictive value of serum HCG concentrations in pregnancies achieved after single fresh or vitrified-warmed blastocyst transfer. Reprod Biomed Online. 2017;35:272–8.
doi: 10.1016/j.rbmo.2017.05.011
Licht P, Russu V, Wildt L. On the role of human chorionic gonadotropin (hCG) in the embryo-endometrial microenvironment: implications for differentiation and implantation. Semin Reprod Med. 2001;19:37–47.
doi: 10.1055/s-2001-13909
Hernandez-Nieto C, Lee JA, Alkon-Meadows T, Luna-Rojas M, Mukherjee T, Copperman AB, Sandler B. Late follicular phase progesterone elevation during ovarian stimulation is not associated with decreased implantation of chromosomally screened embryos in thaw cycles. Hum Reprod. 2020;35(8):1889–99. https://doi.org/10.1093/humrep/deaa123 .
doi: 10.1093/humrep/deaa123
pubmed: 32649755
Veeck LL, Bodine R, Clarke RN, Berrios R, Libraro J, Moschini RM, Zaninovic N, Rosenwaks Z. High pregnancy rates can be achieved after freezing and thawing human blastocysts. Fertil Steril. 2004;82(5):1418–27. https://doi.org/10.1016/j.fertnstert.2004.03.068 .
doi: 10.1016/j.fertnstert.2004.03.068
pubmed: 15533370
Hernandez-Nieto C, Lee JA, Slifkin R, Sandler B, Copperman AB, Flisser E. What is the reproductive potential of day 7 euploid embryos? Hum Reprod. 2019;34(9):1697–706. https://doi.org/10.1093/humrep/dez129 .
doi: 10.1093/humrep/dez129
pubmed: 31398251
Zegers-Hochschild F, Adamson GD, Dyer S, Racowsky C, de Mouzon J, Sokol R, Rienzi L, Sunde A, Schmidt L, Cooke ID, Simpson JL, van der Poel S. The International Glossary on Infertility and Fertility Care, 2017. Hum Reprod. 2017;32(9):1786–801. https://doi.org/10.1093/humrep/dex234 .
doi: 10.1093/humrep/dex234
pubmed: 29117321
pmcid: 5850297
Al Mamari N, Al Zawawi N, Khayat S, Badeghiesh A, Son WY, Dahan MH. Revisiting serum β-hCG cut-off levels and pregnancy outcomes using single embryo transfer. J Assist Reprod Genet. 2019;36(11):2307–13. https://doi.org/10.1007/s10815-019-01583-x (Epub 2019 Oct 12).
doi: 10.1007/s10815-019-01583-x
pubmed: 31605261
pmcid: 6885463
Hoshina M, Boothby M, Hussa R, Patillo R, Camel HM, Boime I. Linkage of human chorionic gonadotrophin and placental lactogen biosynthesis to trophoblast differentiation and tumorigenesis. Placenta. 1985;6:163–72.
doi: 10.1016/S0143-4004(85)80066-7
Shi QJ, Lei ZM, Rao CV, Lin J. Novel role of human chorionic gonadotropin in differentiation of human cytotrophoblasts. Endocrinology. 1993;132:1387–95.
doi: 10.1210/endo.132.3.7679981
Butler SA, Luttoo J, Freire MO, Abban TK, Borrelli PT, Iles RK. Human chorionic gonadotropin (hCG) in the secretome of cultured embryos: hyperglycosylated hCG and hCG-free beta subunit are potential markers for infertility management and treatment. Reprod Sci. 2013;20(9):1038–45. https://doi.org/10.1177/1933719112472739 (Epub 2013 Feb 25).
doi: 10.1177/1933719112472739
pubmed: 23439616
Hill MJ, Richter KS, Heitmann RJ, Graham JR, Tucker MJ, DeCherney AH, Browne PE, Levens ED. Trophectoderm grade predicts outcomes of single-blastocyst transfers. Fertil Steril. 2013;99(5):1283-1289.e1. https://doi.org/10.1016/j.fertnstert.2012.12.003 .
doi: 10.1016/j.fertnstert.2012.12.003
pubmed: 23312233
Dokras A, Sargent IL, Gardner RL, Barlow DH. Human trophectoderm biopsy and secretion of chorionic gonadotrophin. Hum Reprod. 1991;6(10):1453–9. https://doi.org/10.1093/oxfordjournals.humrep.a137288 .
doi: 10.1093/oxfordjournals.humrep.a137288
pubmed: 1770144
Sites CK, St Marie P, Rahil T. Implantation of fresh and thawed-warmed embryos in single embryo transfer cycles: interpreting the initial beta-HCG. Reprod Biomed Online. 2015;30(3):319–21. https://doi.org/10.1016/j.rbmo.2014.12.003 .
doi: 10.1016/j.rbmo.2014.12.003
pubmed: 25592975
Lu MM, Wen YX, Liu YL, Ding CH, Zhou CQ, Xu YW. Trophectoderm biopsy reduces the level of serum β-human chorionic gonadotropin in early pregnancy. Fertil Steril. 2020;114(4):801–8. https://doi.org/10.1016/j.fertnstert.2020.05.015 (Epub 2020 Jul 31).
doi: 10.1016/j.fertnstert.2020.05.015
pubmed: 32741620
Ahlström A, Westin C, Reismer E, Wikland M, Hardarson T. Trophectoderm morphology: an important parameter for predicting live birth after single blastocyst transfer. Hum Reprod. 2011;26(12):3289–96. https://doi.org/10.1093/humrep/der325 .
doi: 10.1093/humrep/der325
pubmed: 21972253
Van den Abbeel E, Balaban B, Ziebe S, Lundin K, Cuesta MJ, Klein BM, Helmgaard L, Arce JC. Association between blastocyst morphology and outcome of single-blastocyst transfer. Reprod Biomed Online. 2013;27(4):353–61. https://doi.org/10.1016/j.rbmo.2013.07.006 .
doi: 10.1016/j.rbmo.2013.07.006
pubmed: 23953585
Poikkeus P, Hiilesmaa V, Tiitinen A. Serum HCG 12 days after embryo transfer in predicting pregnancy outcome. Hum Reprod. 2002;17(7):1901–5. https://doi.org/10.1093/humrep/17.7.1901 .
doi: 10.1093/humrep/17.7.1901
pubmed: 12093858
Ochsenkühn R, Arzberger A, von Schönfeldt V, Engel J, Thaler CJ, Noss U. Predictive value of early serum beta-hCG levels after single blastocyst transfer. Acta Obstet Gynecol Scand. 2009;88(12):1382–8. https://doi.org/10.3109/00016340903322743 .
doi: 10.3109/00016340903322743
pubmed: 19878087
Stenman UH, Alfthan H, Ranta T, Vartiainen E, Jalkanen J, Seppälä M. Serum levels of human chorionic gonadotropin in nonpregnant women and men are modulated by gonadotropin-releasing hormone and sex steroids. J Clin Endocrinol Metab. 1987;64(4):730–6. https://doi.org/10.1210/jcem-64-4-730 .
doi: 10.1210/jcem-64-4-730
pubmed: 3546353
Wang Q, Zhang R, Jia M, Luo L, Ding C, Li TC, Zhou C. Serum human chorionic gonadotropin measured 7 days following day 3 embryo transfer might predict pregnancy outcome in IVF. Gynecol Endocrinol. 2017;33(1):62–6. https://doi.org/10.1080/09513590.2016.1203894 .
doi: 10.1080/09513590.2016.1203894
pubmed: 27449819
Snyder JA, Haymond S, Parvin CA, Gronowski AM, Grenache DG. Diagnostic considerations in the measurement of human chorionic gonadotropin in aging women. Clin Chem. 2005;51(10):1830–5. https://doi.org/10.1373/clinchem.2005.053595 (Epub 2005 Aug 11).
doi: 10.1373/clinchem.2005.053595
pubmed: 16099935
Cole LA, DuToit S, Higgins TN. Total hCG tests. Clin Chim Acta. 2011;412(23–24):2216–22. https://doi.org/10.1016/j.cca.2011.08.006 .
doi: 10.1016/j.cca.2011.08.006
pubmed: 21864517
Cole LA. Immunoassay of human chorionic gonadotropin, its free subunits, and metabolites. Clin Chem. 1997;43(12):2233–43 (PMID: 9439438).
doi: 10.1093/clinchem/43.12.2233
Braunstein GD. False-positive serum human chorionic gonadotropin results: causes, characteristics, and recognition. Am J Obstet Gynecol. 2002;187(1):217–24. https://doi.org/10.1067/mob.2002.124284 .
doi: 10.1067/mob.2002.124284
pubmed: 12114913
Montagnana M, Trenti T, Aloe R, Cervellin G, Lippi G. Human chorionic gonadotropin in pregnancy diagnostics. Clin Chim Acta. 2011;412(17–18):1515–20. https://doi.org/10.1016/j.cca.2011.05.025 .
doi: 10.1016/j.cca.2011.05.025
pubmed: 21635878
Herlihy N, Sekhon L, Nazem T, Hernandez-Nieto C, Oliva M, Lee J, Sandler B, Mukherjee T, Copperman A. Does morphologic grading of embryonic trophectoderm correlate with quality of placentation and perinatal outcome. Fertil Steril, (2017) 108.