Quantification of three antineoplastic agents in urine using the UniSpray ionisation source.

Antineoplastic Agents / urine Chromatography, High Pressure Liquid / methods Chromatography, Liquid Cyclophosphamide Humans Ifosfamide / urine Paclitaxel Tandem Mass Spectrometry / methods
Antineoplastic drug Biomonitoring Liquid chromatography-mass spectrometry Method validation Occupational hygiene UniSpray ionization

Journal

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554

Informations de publication

Date de publication:
01 Aug 2022
Historique:
received: 14 03 2022
revised: 27 05 2022
accepted: 06 06 2022
pubmed: 26 6 2022
medline: 20 7 2022
entrez: 25 6 2022
Statut: ppublish

Résumé

Many guidelines and safety measures led to a decrease in exposure to antineoplastic agents. Since healthcare workers are often exposed to lower concentrations than patients, a sensitive method is needed to quantify occupational exposure. The aim of this study was to develop and validate a sensitive method for simultaneous detection and quantification of cyclophosphamide, ifosfamide and paclitaxel in urine by use of UPLC-MS/MS with a UniSpray ionisation source. Compounds were extracted from urine using Novum simplified liquid extraction cartridges, separated on a C18 column, ionised by a UniSpray ionisation source and detected with MS/MS. In the second part of the study, a field study was performed to assess occupational exposure to antineoplastic agents. Eighty-three samples from healthcare workers were analysed and resulted in seventeen samples containing quantifiable concentrations of at least one compound. In conclusion, a sensitive method for simultaneous detection and quantification of cyclophosphamide (LLOQ 0.05 ng/mL), ifosfamide (LLOQ 0.3 ng/mL) and paclitaxel (LLOQ 0.7 ng/mL) was developed and validated.

Sections du résumé

BACKGROUND BACKGROUND
Many guidelines and safety measures led to a decrease in exposure to antineoplastic agents. Since healthcare workers are often exposed to lower concentrations than patients, a sensitive method is needed to quantify occupational exposure.
OBJECTIVE OBJECTIVE
The aim of this study was to develop and validate a sensitive method for simultaneous detection and quantification of cyclophosphamide, ifosfamide and paclitaxel in urine by use of UPLC-MS/MS with a UniSpray ionisation source.
METHODS METHODS
Compounds were extracted from urine using Novum simplified liquid extraction cartridges, separated on a C18 column, ionised by a UniSpray ionisation source and detected with MS/MS. In the second part of the study, a field study was performed to assess occupational exposure to antineoplastic agents.
RESULTS RESULTS
Eighty-three samples from healthcare workers were analysed and resulted in seventeen samples containing quantifiable concentrations of at least one compound. In conclusion, a sensitive method for simultaneous detection and quantification of cyclophosphamide (LLOQ 0.05 ng/mL), ifosfamide (LLOQ 0.3 ng/mL) and paclitaxel (LLOQ 0.7 ng/mL) was developed and validated.

Identifiants

DOI: 10.1016/j.jchromb.2022.123331 PMID: 35752139
pubmed: 35752139
pii: S1570-0232(22)00235-5
doi: 10.1016/j.jchromb.2022.123331
pii:
doi:

Substances chimiques

Antineoplastic Agents 0
Cyclophosphamide 8N3DW7272P
Paclitaxel P88XT4IS4D
Ifosfamide UM20QQM95Y

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

123331

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Eline Verscheure (E)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Matteo Creta (M)

Unit Environmental Hygiene and Human Biological Monitoring, Department of Health Protection, Laboratoire National de Santé (LNS), Dudelange, Luxembourg.

Dorian Vanneste (D)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Jeroen Vanoirbeek (J)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Meziane Zakia (M)

Centre Hospitalo-universitaire, Service Médicine du Travail, Université Abou Bekr Belkaid Tlemcen, Tlemcen, Algeria; Faculty of Medicine, TOXICOMED Research Laboratory, Université Abou Bekr Belkaid Tlemcen, Tlemcen, Algeria.

Taleb Abdesselam (T)

Centre Hospitalo-universitaire, Service Médicine du Travail, Université Abou Bekr Belkaid Tlemcen, Tlemcen, Algeria; Faculty of Medicine, TOXICOMED Research Laboratory, Université Abou Bekr Belkaid Tlemcen, Tlemcen, Algeria.

Robin Lebegge (R)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Katrien Poels (K)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Manosij Ghosh (M)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Radu-Corneliu Duca (RC)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium; Unit Environmental Hygiene and Human Biological Monitoring, Department of Health Protection, Laboratoire National de Santé (LNS), Dudelange, Luxembourg.

Lode Godderis (L)

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium; Idewe, External Service for Prevention and Protection at Work, Heverlee, Belgium. Electronic address: lode.godderis@kuleuven.be.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Antinéoplasiques › Quantification of three antineoplastic agents...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Quantification of three antineoplastic agents...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Quantification of three antineoplastic agents...
questionsmedicales.fr › Composés chimiques organiques › Composés organiques du phosphore › Phosphoramides › Moutardes phosphoramide › Cyclophosphamide › Quantification of three antineoplastic agents...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Quantification of three antineoplastic agents...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Oxazines › Ifosfamide › Quantification of three antineoplastic agents...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Terpènes › Diterpènes › Taxoïdes › Paclitaxel › Quantification of three antineoplastic agents...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Quantification of three antineoplastic agents...