Methionine oxidation activates pyruvate kinase M2 to promote pancreatic cancer metastasis.


Journal

Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571

Informations de publication

Date de publication:
18 08 2022
Historique:
received: 07 10 2021
revised: 06 04 2022
accepted: 02 06 2022
pubmed: 26 6 2022
medline: 24 8 2022
entrez: 25 6 2022
Statut: ppublish

Résumé

Cancer mortality is primarily a consequence of its metastatic spread. Here, we report that methionine sulfoxide reductase A (MSRA), which can reduce oxidized methionine residues, acts as a suppressor of pancreatic ductal adenocarcinoma (PDA) metastasis. MSRA expression is decreased in the metastatic tumors of PDA patients, whereas MSRA loss in primary PDA cells promotes migration and invasion. Chemoproteomic profiling of pancreatic organoids revealed that MSRA loss results in the selective oxidation of a methionine residue (M239) in pyruvate kinase M2 (PKM2). Moreover, M239 oxidation sustains PKM2 in an active tetrameric state to promote respiration, migration, and metastasis, whereas pharmacological activation of PKM2 increases cell migration and metastasis in vivo. These results demonstrate that methionine residues can act as reversible redox switches governing distinct signaling outcomes and that the MSRA-PKM2 axis serves as a regulatory nexus between redox biology and cancer metabolism to control tumor metastasis.

Identifiants

pubmed: 35752173
pii: S1097-2765(22)00541-X
doi: 10.1016/j.molcel.2022.06.005
pmc: PMC9391305
mid: NIHMS1816254
pii:
doi:

Substances chimiques

Carrier Proteins 0
Membrane Proteins 0
Thyroid Hormones 0
Methionine AE28F7PNPL
Methionine Sulfoxide Reductases EC 1.8.4.-
Pyruvate Kinase EC 2.7.1.40

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3045-3060.e11

Subventions

Organisme : NCI NIH HHS
ID : R01 CA267870
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA036727
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA240654
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013696
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM139245
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA127297
Pays : United States
Organisme : NCI NIH HHS
ID : R50 CA211462
Pays : United States
Organisme : NIH HHS
ID : S10 OD021801
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA210240
Pays : United States

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests C.J.C., F.D.T., and A.H.C. are inventors on patent applications related to the redox-active reagents for methionine conjugation. C.J.T. is listed as an inventor on patents related to PKM2 activators. The remaining authors declare no competing interests.

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Auteurs

Dan He (D)

Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.

Huijin Feng (H)

Institute for Cancer Genetics, Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA.

Belen Sundberg (B)

Institute for Cancer Genetics, Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA.

Jiaxing Yang (J)

Institute for Cancer Genetics, Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA.

Justin Powers (J)

Institute for Cancer Genetics, Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA.

Alec H Christian (AH)

Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.

John E Wilkinson (JE)

University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Cian Monnin (C)

Metabolomics Innovation Resource, Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada.

Daina Avizonis (D)

Metabolomics Innovation Resource, Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada.

Craig J Thomas (CJ)

Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA; Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Richard A Friedman (RA)

Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA; Department of Biomedical Informatics, Columbia University Irving Medical Center, New York, NY 10032, USA.

Michael D Kluger (MD)

Division of Gastrointestinal & Endocrine Surgery, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Michael A Hollingsworth (MA)

Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Paul M Grandgenett (PM)

Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Kelsey A Klute (KA)

Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68198, USA.

F Dean Toste (FD)

Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.

Christopher J Chang (CJ)

Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: chrischang@berkeley.edu.

Iok In Christine Chio (IIC)

Institute for Cancer Genetics, Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA. Electronic address: christine.chio@columbia.edu.

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Classifications MeSH