Ladder observation of bovine serum albumin by high resolution agarose native gel electrophoresis.
MetaPhor agarose
Sieving
UltraPure agarose
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
31 Aug 2022
31 Aug 2022
Historique:
received:
29
03
2022
revised:
16
06
2022
accepted:
16
06
2022
pubmed:
26
6
2022
medline:
4
8
2022
entrez:
25
6
2022
Statut:
ppublish
Résumé
A commercially available bovine serum albumin (BSA) was examined by agarose native gel electrophoresis using two different agarose sources, UltraPure and MetaPhor agarose. While UltraPure agarose up to 5 % showed no clear separation of BSA oligomers, MetaPhor agarose clearly demonstrated oligomer bands above 4 %, indicating that the latter agarose has greater molecular sieving effects and is hence characterized to have high resolution for size differences, as probed by a greater slope of Ferguson plot. Physical properties are different between two agaroses. In general, UltraPure agarose has physical strength, while MetaPhor agarose is considerably fragile, but MetaPhor agarose solution is less viscous so that even 10 % gel can be made. Cause of oligomers was shown to be not associated with inter-chain disulfide bonds, but is due to association of native or native-like molecules.
Identifiants
pubmed: 35752339
pii: S0141-8130(22)01331-9
doi: 10.1016/j.ijbiomac.2022.06.118
pii:
doi:
Substances chimiques
Serum Albumin, Bovine
27432CM55Q
Sepharose
9012-36-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
512-520Informations de copyright
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