Highly selective titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles for the enrichment of intact phosphoproteins.
O-phospho-L-tyrosine
enrichment
magnetic separation
phosphoprotein
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
17
06
2022
received:
29
04
2022
accepted:
20
06
2022
pubmed:
28
6
2022
medline:
18
8
2022
entrez:
27
6
2022
Statut:
ppublish
Résumé
Phosphorylation is one of the most important protein post-translational modifications, which possesses dramatic regulatory effects on the function of proteins. In consideration of the low abundance and low stoichiometry of phosphorylation and non-specific signal suppression, efficient capture of the phosphoproteins from complex biological samples is critical to meet the need for protein profiling. In this work, a facile preparation of titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles was developed for the enrichment of intact phosphoproteins. The prepared magnetic nanoparticles were characterized by various instruments and had a spherical shape with an average diameter of 300 nm. The adsorption isotherms were investigated and the maximum capacity for β-casein was calculated to be 961.5 mg/g. Standard protein mixtures and biological samples (non-fat milk and human serum) were selected to test the enrichment performance. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated the excellent enrichment performance with high selectivity. With the superparamagnetic property, titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles were convenient for the practical application and clinical promotion, thus having a promising prospect in the field of phosphoprotein research.
Identifiants
pubmed: 35754361
doi: 10.1002/jssc.202200351
doi:
Substances chimiques
Caseins
0
Magnetite Nanoparticles
0
Phosphopeptides
0
Phosphoproteins
0
O-phospho-L-tyrosine
2R86C98KDX
Tyrosine
42HK56048U
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3054-3062Subventions
Organisme : National Natural Science Foundation of China
ID : 81772941
Organisme : Key Program of the National Natural Science Foundation
ID : 81930066
Informations de copyright
© 2022 Wiley-VCH GmbH.
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