Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study.
HTLV-1
HTLV-2
molecular diagnosis
multiplex real time PCR
real time PCR
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2022
2022
Historique:
received:
22
02
2022
accepted:
09
05
2022
entrez:
27
6
2022
pubmed:
28
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
Human T cell lymphotropic virus (HTLV) is the caustive agent of two main conditions i. e., the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T-cell leukemia/lymphoma (ATLL). HTLV diagnosis is based on serological and molecular approaches; however, an accurate and validated method is still needed. The objective of this study was to establish a rapid and sensitive molecular test to confirm and discriminate HTLV 1/2 types. The test validation was performed as a multicentric study involving HTLV confirmation centers throughout Brazil. Proviral DNA was extracted from whole blood and the amplification was performed using in-house designed primer and probe sets targeting the
Identifiants
pubmed: 35755037
doi: 10.3389/fmed.2022.881630
pmc: PMC9218175
doi:
Types de publication
Journal Article
Langues
eng
Pagination
881630Informations de copyright
Copyright © 2022 Rocha-Junior, Rodrigues, Slavov, Assone, Pedreschi, de La Roque, Sousa, Olavarria, Galvão-Castro, Fonseca, Penalva de Oliveira, Smid, Takayanagui, Casseb, Covas and Kashima.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Virol. 1998 May;72(5):4327-40
pubmed: 9557723
J Cell Physiol. 2019 Aug;234(8):12433-12441
pubmed: 30633358
J Clin Microbiol. 2009 Apr;47(4):1129-35
pubmed: 19213697
Front Microbiol. 2012 Nov 15;3:388
pubmed: 23162541
J Gen Virol. 1996 Jun;77 ( Pt 6):1193-201
pubmed: 8683207
J Neurovirol. 1997 May;3 Suppl 1:S50-1
pubmed: 9179793
Virus Res. 2008 Jul;135(1):22-5
pubmed: 18343520
Clin Microbiol Rev. 2000 Oct;13(4):559-70
pubmed: 11023957
Retrovirology. 2005 May 09;2:30
pubmed: 15882466
Rev Soc Bras Med Trop. 2010 Mar-Apr;43(2):111-5
pubmed: 20464136
J Virol Methods. 2002 Apr;102(1-2):37-51
pubmed: 11879691
J Clin Virol. 2011 Sep;52(1):38-44
pubmed: 21704554
AIDS Res Hum Retroviruses. 1995 Jul;11(7):813-21
pubmed: 7546908
J Clin Microbiol. 2009 Nov;47(11):3682-91
pubmed: 19741085
Lancet. 1985 Aug 24;2(8452):407-10
pubmed: 2863442
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7994-9
pubmed: 15911757
Transfusion. 2002 Jun;42(6):780-91
pubmed: 12147033
Cad Saude Publica. 2005 May-Jun;21(3):926-31
pubmed: 15868051
Rev Inst Med Trop Sao Paulo. 2012 May-Jun;54(3):123-9
pubmed: 22634882
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415-9
pubmed: 6261256
AIDS Res Hum Retroviruses. 2013 Feb;29(2):359-64
pubmed: 22894552
J Virol Methods. 1998 Nov;75(2):123-40
pubmed: 9870588
Mol Cell Probes. 2003 Apr-Jun;17(2-3):59-68
pubmed: 12788026
J Neurovirol. 2022 Feb;28(1):27-34
pubmed: 35025066
Science. 1982 Nov 5;218(4572):571-3
pubmed: 6981847
Rinsho Ketsueki. 1979 Sep;20(9):1036-9
pubmed: 316015
Lancet. 1990 Dec 1;336(8727):1345-7
pubmed: 1978165
J Infect Dis. 2006 Feb 1;193(3):427-37
pubmed: 16388491
Lancet. 1989 Feb 25;1(8635):441
pubmed: 2563817