Identification of G-quadruplex anti-Interleukin-2 aptamer with high specificity through SELEX stringency.
Aptamer
Dot blot assay
G-quadruplex
Interleukin-2
SELEX stringency
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
22
10
2021
revised:
11
01
2022
accepted:
09
06
2022
entrez:
27
6
2022
pubmed:
28
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
Aptamers are short single-stranded oligonucleotides capable of binding to various targets with high specificity and affinity. This study aimed to identify an aptamer against mouse interleukin-2 (mIL-2) as one of the most important cytokines in autoimmune diseases for diagnostic and therapeutic purposes. For this purpose, 14 SELEX rounds were performed on recombinant mIL-2 with high stringency. The dot blot and flow cytometry techniques were conducted to determine affinity, dissociation constant (K
Identifiants
pubmed: 35756119
doi: 10.1016/j.heliyon.2022.e09721
pii: S2405-8440(22)01009-X
pmc: PMC9218155
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e09721Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Biopolymers. 2000-2001;56(3):147-94
pubmed: 11745110
Mol Biosyst. 2015 May;11(5):1260-70
pubmed: 25720604
Nat Med. 2018 Jul;24(7):1005-1014
pubmed: 29942088
Int Immunopharmacol. 2016 Jul;36:324-332
pubmed: 27232653
Int J Pharm. 2020 Jun 30;584:119403
pubmed: 32387307
Biotechnol Prog. 2021 Sep;37(5):e3191
pubmed: 34218531
Mol Ther. 2017 Jan 4;25(1):54-61
pubmed: 28129128
Methods Mol Biol. 2009;535:19-32
pubmed: 19377986
Clin Transl Oncol. 2017 Aug;19(8):976-988
pubmed: 28224267
Biomaterials. 2020 Feb;230:119626
pubmed: 31753473
Mol Cell Biochem. 2007 Dec;306(1-2):71-7
pubmed: 17660953
Molecules. 2019 Oct 21;24(20):
pubmed: 31640176
Biochim Biophys Acta Gen Subj. 2018 Jul 27;1862(10):2323-2329
pubmed: 30059712
Br J Cancer. 2019 Jan;120(1):6-15
pubmed: 30413827
Int J Mol Sci. 2020 Oct 07;21(19):
pubmed: 33036411
Nucleic Acids Res. 2003 Sep 15;31(18):e110
pubmed: 12954786
Biomolecules. 2021 Dec 02;11(12):
pubmed: 34944461
Front Chem. 2016 May 10;4:14
pubmed: 27242994
J Clin Microbiol. 2013 Jan;51(1):46-54
pubmed: 23077124
Hum Gene Ther Methods. 2019 Feb;30(1):1-16
pubmed: 30700146
J Virol Methods. 2013 May;189(2):362-9
pubmed: 23523887
Biochemistry. 1993 Mar 2;32(8):1899-904
pubmed: 8448147
Tuberculosis (Edinb). 2017 May;104:70-78
pubmed: 28454652
Nat Rev Immunol. 2018 Jul;18(7):454-466
pubmed: 29626211
ACS Omega. 2021 Apr 13;6(16):11005-11014
pubmed: 34056254
Science. 1990 Aug 3;249(4968):505-10
pubmed: 2200121
Anal Methods. 2020 Aug 14;12(30):3823-3835
pubmed: 32676627
J Pharm Biomed Anal. 2014 Mar;91:151-9
pubmed: 24463043
J Biol Eng. 2020 Jan 13;14:1
pubmed: 31956340
Saudi J Biol Sci. 2021 Sep;28(9):5081-5093
pubmed: 34466086
Front Immunol. 2018 Jul 26;9:1683
pubmed: 30093902
Front Chem. 2020 Oct 29;8:570528
pubmed: 33195056
Curr Pharm Des. 2012;18(14):2014-26
pubmed: 22376117
Sci Rep. 2021 Jun 1;11(1):11406
pubmed: 34075115
Transl Res. 2017 May;183:104-120.e2
pubmed: 28087261
Front Bioeng Biotechnol. 2020 May 05;8:401
pubmed: 32432100
Sci Rep. 2019 May 31;9(1):8142
pubmed: 31148584
Analyst. 2020 Jul 21;145(14):4991-5003
pubmed: 32519701
Eur J Biochem. 2000 Jun;267(12):3685-94
pubmed: 10848986
Mol Ther Nucleic Acids. 2014 Aug 26;3:e190
pubmed: 25158092