Methodological considerations for ghrelin isoforms assay in clinical evaluation in anorexia nervosa.
Anorexia nervosa
Appetite
Ghrelin
Immunoassay
Pulsatile secretion
Journal
Comprehensive psychoneuroendocrinology
ISSN: 2666-4976
Titre abrégé: Compr Psychoneuroendocrinol
Pays: England
ID NLM: 101774169
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
17
02
2022
revised:
30
04
2022
accepted:
02
05
2022
entrez:
27
6
2022
pubmed:
28
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
The growing interest concerning the role of metabolic sensors in various eating disorders requires the implementation of a strict methodology to collect, store and process blood samples in clinical studies. In particular, measurement of isoforms of the appetite-stimulating hormone, ghrelin, has been challenging in clinical settings. Indeed the acyl ghrelin (AG) isoform is rapidly degraded into desacyl ghrelin (DAG) by blood esterases, thus optimal conditions for the conservation of AG and accurate determination of AG/DAG ratio should be used. Here, we compared different protease inhibitors (Aprotinin, PHMB, AEBSF) during blood collection, increasing delays (0-180 min) before centrifugation, plasma supplementation with various HCl concentrations, storage durations of frozen plasma (8 and 447 days) and immunoenzyme-assay procedures (one-step
Identifiants
pubmed: 35757178
doi: 10.1016/j.cpnec.2022.100140
pii: S2666-4976(22)00031-5
pmc: PMC9227986
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100140Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Benoît de Grimaudet is working at Bertin Biotechnologies.
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