Engagement of TRAIL triggers degranulation and IFNγ production in human natural killer cells.


Journal

EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049

Informations de publication

Date de publication:
03 08 2022
Historique:
revised: 16 05 2022
received: 10 10 2021
accepted: 23 05 2022
pubmed: 28 6 2022
medline: 5 8 2022
entrez: 27 6 2022
Statut: ppublish

Résumé

NK cells utilize a large array of receptors to screen their surroundings for aberrant or virus-infected cells. Given the vast diversity of receptors expressed on NK cells we seek to identify receptors involved in the recognition of HIV-1-infected cells. By combining an unbiased large-scale screening approach with a functional assay, we identify TRAIL to be associated with NK cell degranulation against HIV-1-infected target cells. Further investigating the underlying mechanisms, we demonstrate that TRAIL is able to elicit multiple effector functions in human NK cells independent of receptor-mediated induction of apoptosis. Direct engagement of TRAIL not only results in degranulation but also IFNγ production. Moreover, TRAIL-mediated NK cell activation is not limited to its cognate death receptors but also decoy receptor I, adding a new perspective to the perceived regulatory role of decoy receptors in TRAIL-mediated cytotoxicity. Based on these findings, we propose that TRAIL not only contributes to the anti-HIV-1 activity of NK cells but also possesses a multifunctional role beyond receptor-mediated induction of apoptosis, acting as a regulator for the induction of different effector functions.

Identifiants

pubmed: 35758160
doi: 10.15252/embr.202154133
pmc: PMC9346491
doi:

Substances chimiques

TNF-Related Apoptosis-Inducing Ligand 0
TNFSF10 protein, human 0
Interferon-gamma 82115-62-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e54133

Informations de copyright

© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

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Auteurs

Johannes Höfle (J)

Leibniz Institute of Virology, Hamburg, Germany.

Timo Trenkner (T)

Leibniz Institute of Virology, Hamburg, Germany.

Nadja Kleist (N)

Leibniz Institute of Virology, Hamburg, Germany.

Vera Schwane (V)

Leibniz Institute of Virology, Hamburg, Germany.

Sarah Vollmers (S)

Leibniz Institute of Virology, Hamburg, Germany.

Bryan Barcelona (B)

Leibniz Institute of Virology, Hamburg, Germany.

Annika Niehrs (A)

Leibniz Institute of Virology, Hamburg, Germany.

Pia Fittje (P)

Leibniz Institute of Virology, Hamburg, Germany.

Van Hung Huynh-Tran (VH)

Inserm, Bordeaux Population Health Research Center, UMR1219 and Inria, team SISTM, University of Bordeaux, Bordeaux, France.

Alexander H Schmidt (AH)

DKMS, Tübingen, Germany.
DKMS Life Science Lab, Dresden, Germany.

Sven Peine (S)

Institute of Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Angelique Hoelzemer (A)

Leibniz Institute of Virology, Hamburg, Germany.
German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, Hamburg, Germany.
First Department of Medicine, Division of Infectious Diseases, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Laura Richert (L)

Inserm, Bordeaux Population Health Research Center, UMR1219 and Inria, team SISTM, University of Bordeaux, Bordeaux, France.

Marcus Altfeld (M)

Leibniz Institute of Virology, Hamburg, Germany.
Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Christian Körner (C)

Leibniz Institute of Virology, Hamburg, Germany.

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