Improved skeletal muscle fatigue resistance in experimental autoimmune myositis mice following high-intensity interval training.
Journal
Arthritis research & therapy
ISSN: 1478-6362
Titre abrégé: Arthritis Res Ther
Pays: England
ID NLM: 101154438
Informations de publication
Date de publication:
27 06 2022
27 06 2022
Historique:
received:
27
04
2022
accepted:
18
06
2022
entrez:
27
6
2022
pubmed:
28
6
2022
medline:
30
6
2022
Statut:
epublish
Résumé
Muscle weakness and decreased fatigue resistance are key manifestations of systemic autoimmune myopathies (SAMs). We here examined whether high-intensity interval training (HIIT) improves fatigue resistance in the skeletal muscle of experimental autoimmune myositis (EAM) mice, a widely used animal model for SAM. Female BALB/c mice were randomly assigned to control (CNT) or EAM groups (n = 28 in each group). EAM was induced by immunization with three injections of myosin emulsified in complete Freund's adjuvant. The plantar flexor (PF) muscles of mice with EAM were exposed to either an acute bout or 4 weeks of HIIT (a total of 14 sessions). The fatigue resistance of PF muscles was lower in the EAM than in the CNT group (P < 0.05). These changes were associated with decreased activities of citrate synthase and cytochrome c oxidase and increased expression levels of the endoplasmic reticulum stress proteins (glucose-regulated protein 78 and 94, and PKR-like ER kinase) (P < 0.05). HIIT restored all these alterations and increased the peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and the mitochondrial electron transport chain complexes (I, III, and IV) in the muscles of EAM mice (P < 0.05). HIIT improves fatigue resistance in a SAM mouse model, and this can be explained by the restoration of mitochondria oxidative capacity via inhibition of the ER stress pathway and PGC-1α-mediated mitochondrial biogenesis.
Sections du résumé
BACKGROUND
Muscle weakness and decreased fatigue resistance are key manifestations of systemic autoimmune myopathies (SAMs). We here examined whether high-intensity interval training (HIIT) improves fatigue resistance in the skeletal muscle of experimental autoimmune myositis (EAM) mice, a widely used animal model for SAM.
METHODS
Female BALB/c mice were randomly assigned to control (CNT) or EAM groups (n = 28 in each group). EAM was induced by immunization with three injections of myosin emulsified in complete Freund's adjuvant. The plantar flexor (PF) muscles of mice with EAM were exposed to either an acute bout or 4 weeks of HIIT (a total of 14 sessions).
RESULTS
The fatigue resistance of PF muscles was lower in the EAM than in the CNT group (P < 0.05). These changes were associated with decreased activities of citrate synthase and cytochrome c oxidase and increased expression levels of the endoplasmic reticulum stress proteins (glucose-regulated protein 78 and 94, and PKR-like ER kinase) (P < 0.05). HIIT restored all these alterations and increased the peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and the mitochondrial electron transport chain complexes (I, III, and IV) in the muscles of EAM mice (P < 0.05).
CONCLUSIONS
HIIT improves fatigue resistance in a SAM mouse model, and this can be explained by the restoration of mitochondria oxidative capacity via inhibition of the ER stress pathway and PGC-1α-mediated mitochondrial biogenesis.
Identifiants
pubmed: 35761371
doi: 10.1186/s13075-022-02846-2
pii: 10.1186/s13075-022-02846-2
pmc: PMC9235155
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
156Informations de copyright
© 2022. The Author(s).
Références
Biochem Med. 1985 Apr;33(2):158-69
pubmed: 4004819
J Physiol. 1978 Feb;275:241-62
pubmed: 24736
Clin Physiol Funct Imaging. 2011 Jan;31(1):18-25
pubmed: 21029327
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Arthritis Rheumatol. 2021 May;73(5):848-857
pubmed: 33191613
Cell Metab. 2013 Feb 5;17(2):162-84
pubmed: 23395166
Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):15492-7
pubmed: 26575622
Arthritis Rheum. 2012 Nov;64(11):3750-9
pubmed: 22806328
Neuromuscul Disord. 1991;1(5):333-9
pubmed: 1822343
Mol Med. 2010 Nov-Dec;16(11-12):455-64
pubmed: 20809047
J Biol Chem. 2005 May 20;280(20):19587-93
pubmed: 15767263
Mol Genet Metab Rep. 2014 Jan 16;1:51-59
pubmed: 27896074
Antioxid Redox Signal. 2014 Jul 20;21(3):396-413
pubmed: 24702237
Curr Rheumatol Rep. 2014 Jul;16(7):429
pubmed: 24879535
Arthritis Rheum. 2005 Jun;52(6):1824-35
pubmed: 15934115
Rheumatology (Oxford). 2009 Feb;48(2):134-9
pubmed: 19074186
Ann Rheum Dis. 1995 Jun;54(6):491-3
pubmed: 7632092
Methods Biochem Anal. 1955;2:427-34
pubmed: 14393574
J Rheumatol. 1993 Aug;20(8):1399-401
pubmed: 8230026
J Physiol. 2006 Aug 1;574(Pt 3):889-903
pubmed: 16690701
Arthritis Res Ther. 2013 Aug 13;15(4):R83
pubmed: 23941324
Arch Phys Med Rehabil. 2000 Jan;81(1):1-5
pubmed: 10638867
Cell Death Dis. 2014 Jan 09;5:e989
pubmed: 24407242
Appl Physiol Nutr Metab. 2018 Oct;43(10):1059-1068
pubmed: 29733694
Diabetologia. 2013 Jun;56(6):1372-82
pubmed: 23460021
Neurology. 1992 Jan;42(1):170-9
pubmed: 1370861
Am J Pathol. 2009 Mar;174(3):989-98
pubmed: 19218348
J Clin Invest. 1984 Mar;73(3):720-30
pubmed: 6707201
J Appl Physiol (1985). 2007 Jul;103(1):315-22
pubmed: 17463303
Am J Physiol Regul Integr Comp Physiol. 2016 Nov 1;311(5):R919-R929
pubmed: 27654397
Arthritis Rheumatol. 2016 Jul;68(7):1738-50
pubmed: 26867141
Diabetes. 2010 Jun;59(6):1386-96
pubmed: 20299472
J Physiol. 1982 Jun;327:79-94
pubmed: 7120151
J Immunol. 2005 Nov 15;175(10):6987-96
pubmed: 16272359
Ann Rheum Dis. 2018 Jan;77(1):40-47
pubmed: 28814428
J Rheumatol. 1993 Aug;20(8):1340-4
pubmed: 8230016
Ann Rheum Dis. 2015 Jul;74(7):1340-6
pubmed: 26063809
FASEB J. 2021 Nov;35(11):e21988
pubmed: 34665879
Adv Rheumatol. 2018 May 24;58(1):5
pubmed: 30657065
J Physiol. 2000 Oct 1;528 Pt 1:221-6
pubmed: 11018120
PLoS One. 2013 Nov 12;8(11):e74450
pubmed: 24265670
J Physiol. 2018 Jul;596(14):2823-2840
pubmed: 29727016
J Physiol. 2012 Mar 1;590(5):1077-84
pubmed: 22289907
Arthritis Rheumatol. 2020 Jul;72(7):1170-1183
pubmed: 32009304
J Appl Physiol (1985). 2013 Sep;115(6):785-93
pubmed: 23788574
Ann Rheum Dis. 2012 Apr;71(4):582-8
pubmed: 22121133
J Physiol. 2018 Sep;596(18):4427-4442
pubmed: 30062729
Curr Opin Rheumatol. 2009 Nov;21(6):599-603
pubmed: 19726993
Cell Death Differ. 2012 Nov;19(11):1880-91
pubmed: 22705852
Acta Neuropathol. 2017 Oct;134(4):655-666
pubmed: 28623559
Acta Physiol Scand. 2004 Jan;180(1):79-87
pubmed: 14706116
Front Physiol. 2018 Aug 10;9:1111
pubmed: 30147660
Antioxidants (Basel). 2020 Aug 05;9(8):
pubmed: 32764412