Genome-wide decrease in DNA methylation in adults with epilepsy treated with modified ketogenic diet: A prospective study.
Infinium MethylationEPIC BeadChip
epigenetics
high-fat
low-carbohydrate diet
Journal
Epilepsia
ISSN: 1528-1167
Titre abrégé: Epilepsia
Pays: United States
ID NLM: 2983306R
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
27
06
2022
received:
28
02
2022
accepted:
27
06
2022
pubmed:
29
6
2022
medline:
21
9
2022
entrez:
28
6
2022
Statut:
ppublish
Résumé
The aim of this study was to investigate the impact of the modified ketogenic diet on DNA methylation in adults with epilepsy. In this prospective study, we investigated the genome-wide DNA methylation in whole blood in 58 adults with epilepsy treated with the modified ketogenic for 12 weeks. Patients were recruited from the National Center for Epilepsy, Norway, from March 1, 2011 to February 28, 2017. DNA methylation was analyzed using the Illumina Infinium MethylationEPIC BeadChip array. Analysis of variance and paired t-test were used to identify differentially methylated loci after 4 and 12 weeks of dietary treatment. A false discovery rate approach with a significance threshold of <5% was used to adjust for multiple comparisons. We observed a genome-wide decrease in DNA methylation, both globally and at specific sites, after 4 and 12 weeks of dietary treatment. A substantial share of the differentially methylated positions (CpGs) were annotated to genes associated with epilepsy (n = 7), lipid metabolism (n = 8), and transcriptional regulation (n = 10). Furthermore, five of the identified genes were related to inositol phosphate metabolism, which may represent a possible mechanism by which the ketogenic diet attenuates seizures. A better understanding of the modified ketogenic diet's influence at the molecular level may be the key to unraveling the mechanisms by which the diet can ameliorate seizures and possibly to identifying novel therapeutic targets for epilepsy.
Identifiants
pubmed: 35762681
doi: 10.1111/epi.17351
pmc: PMC9796519
doi:
Substances chimiques
Inositol Phosphates
0
Ketone Bodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2413-2426Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022 The Authors. Epilepsia published by Wiley Periodicals LLC on behalf of International League Against Epilepsy.
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