Involvement of ferroptosis in Porphyromonas gingivalis lipopolysaccharide-stimulated periodontitis in vitro and in vivo.
Porphyromonas gingivalis
ferroptosis
fibroblast
lipopolysaccharide
periodontitis
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
11
05
2022
received:
31
10
2021
accepted:
24
06
2022
medline:
7
12
2023
pubmed:
30
6
2022
entrez:
29
6
2022
Statut:
ppublish
Résumé
Ferroptosis is associated with multiple inflammatory diseases. Periodontitis is an inflammatory disease mainly caused by oral opportunistic pathogens. However, the ferroptosis-periodontitis relationship has not been thoroughly described. We here analyzed whether ferroptosis is involved in periodontitis. Human gingival fibroblasts (HGFs) were stimulated with P. gingivalis-LPS and ferrostatin-1 (Fer-1, a ferroptosis inhibitor), and changes in mitochondrial morphology, ferroptosis-related factors, and inflammation levels were detected. After the rat experimental periodontitis model was established, changes in ferroptosis-related factors and inflammation levels were re-evaluated in the same manner. Porphyromonas gingivalis-LPS-induced mitochondrial shrinkage, an increase in mitochondrial membrane density, and upregulation of reactive oxygen species in HGFs. The expression of prostaglandin-endoperoxide synthase 2, transferrin receptor 1, and malondialdehyde and inflammation levels were upregulated, whereas the expression of solute carrier family seven member 11, glutathione peroxidase 4, superoxide dismutase, and glutathione were downregulated. Fer-1 attenuated these aforementioned changes and inflammation levels induced by P. gingivalis-LPS. The in vivo experiment results were consistent with the in vitro experiment results. Ferroptosis is involved in inflammatory processes in HGFs upon P. gingivalis-LPS stimulation. Ferroptosis is observed in the gingival tissue of periodontitis rats.
Substances chimiques
Lipopolysaccharides
0
Types de publication
Journal Article
Langues
eng
Pagination
3571-3582Subventions
Organisme : Department of Finance of Jilin Province
ID : JCSZ2019378-3
Organisme : Department of Finance of Jilin Province
ID : JCSZ2020304-15
Organisme : Department of Finance of Jilin Province
ID : JCSZ2020304-24
Organisme : Department of Science and Technology of Jilin Province
ID : 20200404108YY
Organisme : Department of Science and Technology of Jilin Province
ID : YDZJ202201ZYTS244
Informations de copyright
© 2022 Wiley Periodicals LLC.
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