Performance of islets of Langerhans conformally coated via an emulsion cross-linking method in diabetic rodents and nonhuman primates.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
entrez:
29
6
2022
pubmed:
30
6
2022
medline:
2
7
2022
Statut:
ppublish
Résumé
Polyethylene glycol (PEG)-based conformal coating (CC) encapsulation of transplanted islets is a promising β cell replacement therapy for the treatment of type 1 diabetes without chronic immunosuppression because it minimizes capsule thickness, graft volume, and insulin secretion delay. However, we show here that our original CC method, the direct method, requiring exposure of islets to low pH levels and inclusion of viscosity enhancers during coating, severely affected the viability, scalability, and biocompatibility of CC islets in nonhuman primate preclinical models of type 1 diabetes. We therefore developed and validated in vitro and in vivo, in several small- and large-animal models of type 1 diabetes, an augmented CC method-emulsion method-that achieves hydrogel CCs around islets at physiological pH for improved cytocompatibility, with PEG hydrogels for increased biocompatibility and with fivefold increase in encapsulation throughput for enhanced scalability.
Identifiants
pubmed: 35767620
doi: 10.1126/sciadv.abm3145
pmc: PMC9242596
doi:
Substances chimiques
Emulsions
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabm3145Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK109929
Pays : United States
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