Separation and detection of D-/L-serine by conventional HPLC.
D-/L-serine
D-amino acid
Detection
Diastereomer method
N-acetyl-L-cysteine (NAC)
Ortho-phthalaldehyde (OPA)
Reversed phase HPLC with ECD
Separation
Journal
MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829
Informations de publication
Date de publication:
2022
2022
Historique:
received:
01
04
2022
accepted:
03
06
2022
entrez:
30
6
2022
pubmed:
1
7
2022
medline:
1
7
2022
Statut:
epublish
Résumé
D-serine has a role as an endogenous allosteric agonist of N-methyl-D-aspartate (NMDA) receptor in the mammalian brain. In this study, we present a detailed description of our method that measures D-/L-serine by using conventional high performance liquid chromatography (HPLC). • We reacted D-serine and L-serine with ortho-phthalaldehyde (OPA) and N-acetyl-L-cysteine (NAC) to form diastereomeric isoindole derivatives, then we separated and detected them by conventional reversed phase HPLC with electrochemical detector (ECD). • We present typical measurement data of rat brain homogenate as an example of a convenient, appropriate method for measuring brain concentrations of D-serine. • Since many peaks appear in biological samples, we confirmed that the peaks were derived from serine by treating the sample with D-amino oxidase and catalase to decompose D-serine. As a results, one peak disappeared, suggesting that it is derived from D-serine.
Identifiants
pubmed: 35769612
doi: 10.1016/j.mex.2022.101752
pii: S2215-0161(22)00132-7
pmc: PMC9234346
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101752Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Proc Jpn Acad Ser B Phys Biol Sci. 2007 Jun;83(5):127-35
pubmed: 24019591
J Chromatogr. 1992 Nov 6;582(1-2):41-8
pubmed: 1491056
Curr Opin Clin Nutr Metab Care. 2013 Jan;16(1):72-5
pubmed: 23041616
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4926-31
pubmed: 10781100
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 1;879(29):3169-83
pubmed: 21992750
J Chromatogr B Biomed Sci Appl. 2001 Jun 5;757(1):119-25
pubmed: 11419736
Neurochem Int. 2013 Dec;63(8):756-64
pubmed: 24140431
Chem Res Toxicol. 2008 Dec;21(12):2272-9
pubmed: 19548351
Eur J Pharmacol. 2022 May 15;923:174930
pubmed: 35364072
ASN Neuro. 2017 May-Jun;9(3):1759091417713905
pubmed: 28604057
Prog Neuropsychopharmacol Biol Psychiatry. 2005 Jun;29(5):767-9
pubmed: 15939521
Neurosci Lett. 2008 Nov 7;445(1):53-7
pubmed: 18775473