Fast and high-fidelity

Animals Imaging, Three-Dimensional / methods Mice Microscopy, Confocal / methods Staining and Labeling Stem Cells
Cancer Cell Biology Microscopy Stem Cells

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
entrez: 30 6 2022
pubmed: 1 7 2022
medline: 2 7 2022
Statut: epublish

Résumé

Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFAST3D) that preserves the 3D structure of the entire tissue and that of subcellular structures with high fidelity. The iFAST3D protocol enables reproducible and high-resolution 3D imaging of stem cells and various niche components for many mouse organs and tissues. For complete details on the use and execution of this protocol, please refer to Saçma et al. (2019).

Identifiants

DOI: 10.1016/j.xpro.2022.101483 PMID: 35769923 PMC: PMC9234157
pubmed: 35769923
doi: 10.1016/j.xpro.2022.101483
pii: S2666-1667(22)00363-X
pmc: PMC9234157
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101483

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Nature. 2015 Oct 1;526(7571):126-30
pubmed: 26416744
Nat Methods. 2018 Jan;15(1):39-46
pubmed: 29320487
Nat Cell Biol. 2013 May;15(5):533-43
pubmed: 23624405
Nature. 2013 Oct 31;502(7473):637-43
pubmed: 24107994
Dev Biol. 2007 Apr 1;304(1):246-59
pubmed: 17239845
Nature. 2014 Mar 20;507(7492):323-328
pubmed: 24646994
J Comp Neurol. 2004 Feb 9;469(3):311-24
pubmed: 14730584
Blood. 2022 Feb 3;139(5):690-703
pubmed: 34657154
STAR Protoc. 2021 Jun 18;2(3):100499
pubmed: 34195671
Nat Cell Biol. 2019 Nov;21(11):1309-1320
pubmed: 31685996

Auteurs

Mehmet Saçma (M)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany. Electronic address: mehmet.sacma@uni-ulm.de.

Francesca Matteini (F)

Stem Cell Aging Group, Regenerative Medicine Program, The Bellvitge Institute for Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain; Program for Advancing the Clinical Translation of Regenerative Medicine of Catalonia, P-CMR[C], L'Hospitalet de Llobregat, Barcelona, Spain.

Medhanie A Mulaw (MA)

Molecular Oncology Institute of Experimental Cancer Research, Medical Faculty, Ulm University, Ulm, Germany.

Ali Hageb (A)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.

Ruzhica Bogeska (R)

Division of Experimental Hematology, German Cancer Research Center (DKFZ) and Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM), Heidelberg, Germany.

Vadim Sakk (V)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.

Angelika Vollmer (A)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.

Gina Marka (G)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.

Karin Soller (K)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.

Michael D Milsom (MD)

Division of Experimental Hematology, German Cancer Research Center (DKFZ) and Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM), Heidelberg, Germany.

Maria Carolina Florian (MC)

Stem Cell Aging Group, Regenerative Medicine Program, The Bellvitge Institute for Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain; Program for Advancing the Clinical Translation of Regenerative Medicine of Catalonia, P-CMR[C], L'Hospitalet de Llobregat, Barcelona, Spain; Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.

Hartmut Geiger (H)

Institute of Molecular Medicine, Ulm University, 89081 Ulm, Germany. Electronic address: hartmut.geiger@uni-ilm.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Fast and high-fidelity
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Fast and high-fidelity
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Fast and high-fidelity
questionsmedicales.fr › Techniques d'investigation › Microscopie confocale › Fast and high-fidelity
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Fast and high-fidelity
questionsmedicales.fr › Cellules › Cellules souches › Fast and high-fidelity