DNA fragmentation of equine cumulus cells from Cumulus-Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte.
DNA fragmentation
cumulus cells
equine
oocyte vitrification
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
received:
03
05
2022
accepted:
28
06
2022
pubmed:
1
7
2022
medline:
5
10
2022
entrez:
30
6
2022
Statut:
ppublish
Résumé
The objectives of this study were to evaluate the effect of vitrification on the DNA fragmentation rate of equine cumulus cells and to assess its relationship to oocyte in vitro maturation (IVM) after vitrification. Cumulus cells (CC) from 14 mares were recovered from COCs, previously submitted to vitrification (VIT) and IVM. The DNA fragmentation rate of the cumulus cells (CC-DF) was assessed using a chromatin dispersion test. CC-DF rates between vitrified and control COCs were statistically compared by Student's t-test. The rates of CC-DF from control COCs were lower than in vitrified COCs. The percentage of CC-DF was not significantly different (p > .05) between groups of COCs able to reach metaphase II (MII > 0) and those in which oocyte maturation was not achieved (MII = 0). In conclusion, vitrification has a deleterious effect on the DNA fragmentation of equine cumulus cells; however, this parameter cannot be used as a predictor for IVM success after COCs vitrification.
Identifiants
pubmed: 35770452
doi: 10.1111/rda.14197
pmc: PMC9795872
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
64-67Subventions
Organisme : Ministerio de Economía y Competitividad
Organisme : European Social Fund 2014-2020
ID : DOC_00399
Informations de copyright
© 2022 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.
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