Convection Driven Ultrarapid Protein Detection via Nanobody-Functionalized Organic Electrochemical Transistors.
SARS-CoV-2
alternating current electrokinetics/electrohydrodynamics
biosensors
nanobodies
organic electrochemical transistors
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
15
06
2022
received:
01
04
2022
pubmed:
1
7
2022
medline:
9
9
2022
entrez:
30
6
2022
Statut:
ppublish
Résumé
Conventional biosensors rely on the diffusion-dominated transport of the target analyte to the sensor surface. Consequently, they require an incubation step that may take several hours to allow for the capture of analyte molecules by sensor biorecognition sites. This incubation step is a primary cause of long sample-to-result times. Here, alternating current electrothermal flow (ACET) is integrated in an organic electrochemical transistor (OECT)-based sensor to accelerate the device operation. ACET is applied to the gate electrode functionalized with nanobody-SpyCatcher fusion proteins. Using the SARS-CoV-2 spike protein in human saliva as an example target, it is shown that ACET enables protein recognition within only 2 min of sample exposure, supporting its use in clinical practice. The ACET integrated sensor exhibits better selectivity, higher sensitivity, and lower limit of detection than the equivalent sensor with diffusion-dominated operation. The performance of ACET integrated sensors is compared with two types of organic semiconductors in the channel and grounds for device-to-device variations are investigated. The results provide guidelines for the channel material choice in OECT-based biochemical sensors, and demonstrate that ACET integration substantially decreases the detection speed while increasing the sensitivity and selectivity of transistor-based sensors.
Identifiants
pubmed: 35772173
doi: 10.1002/adma.202202972
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2202972Subventions
Organisme : King Abdullah University of Science and Technology
Organisme : Office of Sponsored Research Competitive Research Grants
ID : OSR-2018-CRG7-3709
Organisme : Office of Sponsored Research Competitive Research Grants
ID : OSR-2019-CRG8-4073
Organisme : Office of Sponsored Research Competitive Research Grants
ID : REI/1/4204-01
Organisme : Office of Sponsored Research Competitive Research Grants
ID : REI/1/4577-01
Organisme : Office of Sponsored Research Competitive Research Grants
ID : URF/1/1976-33-01
Organisme : KAUST
Organisme : Office of Sponsored Research
ID : OSR-2018-CRG/CCF-3079
Organisme : Office of Sponsored Research
ID : OSR-2019-CRG8-4086
Organisme : Office of Sponsored Research
ID : OSR-2018-CRG7-3749
Organisme : ERC
ID : 610115
Organisme : European Union's Horizon 2020 Research and Innovation Programme
ID : 952911
Organisme : European Union's Horizon 2020 Research and Innovation Programme
ID : 862474
Organisme : EPSRC
ID : EP/T026219/1
Organisme : NSF
ID : DMR-1751308
Organisme : H2020 European Research Council
ID : 952911
Informations de copyright
© 2022 Wiley-VCH GmbH.
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