Crystal structure of Trypanosoma cruzi heme peroxidase and characterization of its substrate specificity and compound I intermediate.
Chagas disease
ascorbate
cytochrome c
heme
oxidants
peroxidase
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
10
05
2022
revised:
21
06
2022
accepted:
22
06
2022
pubmed:
1
7
2022
medline:
9
9
2022
entrez:
30
6
2022
Statut:
ppublish
Résumé
The protozoan parasite Trypanosoma cruzi is the causative agent of American trypanosomiasis, otherwise known as Chagas disease. To survive in the host, the T. cruzi parasite needs antioxidant defense systems. One of these is a hybrid heme peroxidase, the T. cruzi ascorbate peroxidase-cytochrome c peroxidase enzyme (TcAPx-CcP). TcAPx-CcP has high sequence identity to members of the class I peroxidase family, notably ascorbate peroxidase (APX) and cytochrome c peroxidase (CcP), as well as a mitochondrial peroxidase from Leishmania major (LmP). The aim of this work was to solve the structure and examine the reactivity of the TcAPx-CcP enzyme. Low temperature electron paramagnetic resonance spectra support the formation of an exchange-coupled [Fe(IV)=O Trp
Identifiants
pubmed: 35772495
pii: S0021-9258(22)00646-9
doi: 10.1016/j.jbc.2022.102204
pmc: PMC9358470
pii:
doi:
Substances chimiques
Antioxidants
0
Heme
42VZT0U6YR
Cytochromes c
9007-43-6
Peroxidases
EC 1.11.1.-
Ascorbate Peroxidases
EC 1.11.1.11
Cytochrome-c Peroxidase
EC 1.11.1.5
Peroxidase
EC 1.11.1.7
Ascorbic Acid
PQ6CK8PD0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102204Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
Références
Science. 1992 Dec 11;258(5089):1748-55
pubmed: 1334573
Anal Biochem. 1972 Oct;49(2):474-8
pubmed: 5082943
Eur J Biochem. 2002 Jul;269(13):3182-92
pubmed: 12084058
Acc Chem Res. 2018 Feb 20;51(2):427-435
pubmed: 29327921
Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):E1326-E1335
pubmed: 28179568
Biochemistry. 2011 Dec 20;50(50):10910-8
pubmed: 22082147
FEBS J. 2012 May;279(9):1632-9
pubmed: 22093282
Science. 2014 Jul 11;345(6193):193-7
pubmed: 25013070
Arch Biochem Biophys. 2002 Jul 1;403(1):141-4
pubmed: 12061811
Biochim Biophys Acta. 2005 Feb 25;1707(1):91-102
pubmed: 15721608
Biochemistry. 1996 May 14;35(19):6107-15
pubmed: 8634253
J Biol Chem. 1999 Dec 31;274(53):37827-33
pubmed: 10608846
Biochemistry. 2012 Mar 27;51(12):2453-60
pubmed: 22372542
Biochemistry. 2001 Dec 18;40(50):15362-8
pubmed: 11735419
Proc Natl Acad Sci U S A. 2019 Apr 30;116(18):8879-8888
pubmed: 30979807
J Exp Med. 2019 Mar 4;216(3):501-516
pubmed: 30792185
Biochemistry. 2000 Aug 22;39(33):10132-9
pubmed: 10956001
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
pubmed: 8744570
Biochemistry. 1993 Apr 27;32(16):4430-43
pubmed: 8386547
Biochemistry. 1999 Apr 27;38(17):5538-45
pubmed: 10220341
Nucleic Acids Res. 2010 Jan;38(Database issue):D457-62
pubmed: 19843604
Trans R Soc Trop Med Hyg. 1986;80(3):462-70
pubmed: 3099437
J Chem Theory Comput. 2015 Aug 11;11(8):3696-713
pubmed: 26574453
Biochemistry. 2008 Dec 30;47(52):13933-41
pubmed: 19061385
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2184-95
pubmed: 15572771
Antioxid Redox Signal. 2013 Sep 1;19(7):723-34
pubmed: 22458250
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13453-8
pubmed: 12351682
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3704-9
pubmed: 8623000
J Am Chem Soc. 2001 May 30;123(21):5050-8
pubmed: 11457334
Clin Microbiol Rev. 2011 Oct;24(4):655-81
pubmed: 21976603
Protein Sci. 2018 Jan;27(1):112-128
pubmed: 28836357
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11645-50
pubmed: 16087875
Subcell Biochem. 2000;35:317-49
pubmed: 11192727
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
J Biol Chem. 2011 Feb 25;286(8):6627-40
pubmed: 21098483
Biochem J. 2018 Apr 5;475(7):1235-1251
pubmed: 29438066
Nucleic Acids Res. 1990 Oct 25;18(20):6097-100
pubmed: 2172928
BMC Bioinformatics. 2004 Aug 19;5:113
pubmed: 15318951
J Comput Chem. 2010 Mar;31(4):797-810
pubmed: 19569205
Mol Biochem Parasitol. 1994 Jul;66(1):143-5
pubmed: 7984177
Nat Prod Rep. 2003 Aug;20(4):367-81
pubmed: 12964833
J Biol Chem. 2011 Jul 15;286(28):24608-15
pubmed: 21566139
Parasite. 2014;21:11
pubmed: 24626257
Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18390-4
pubmed: 23100535
Biochemistry. 2002 Nov 19;41(46):13774-81
pubmed: 12427040
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Biochemistry. 1995 Apr 4;34(13):4342-5
pubmed: 7703248
J Am Chem Soc. 2011 Jun 15;133(23):9052-62
pubmed: 21548577
Arch Biochem Biophys. 2002 Jan 1;397(1):57-68
pubmed: 11747310
Biochemistry. 2008 Mar 4;47(9):2766-75
pubmed: 18232645
Biochemistry. 2008 Sep 16;47(37):9781-92
pubmed: 18712881
Biochemistry. 2006 Jun 27;45(25):7808-17
pubmed: 16784232
Nat Commun. 2016 Nov 29;7:13445
pubmed: 27897163
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Nat Struct Biol. 2003 Apr;10(4):303-7
pubmed: 12640445
Biochem J. 2005 Sep 1;390(Pt 2):465-74
pubmed: 15850459
Science. 1989 Aug 18;245(4919):738-40
pubmed: 2549632