Exosomal miR-145 and miR-885 Regulate Thrombosis in COVID-19.
Journal
The Journal of pharmacology and experimental therapeutics
ISSN: 1521-0103
Titre abrégé: J Pharmacol Exp Ther
Pays: United States
ID NLM: 0376362
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
14
03
2022
accepted:
09
05
2022
pmc-release:
01
01
2024
pubmed:
1
7
2022
medline:
27
12
2022
entrez:
30
6
2022
Statut:
ppublish
Résumé
We hypothesized that exosomal microRNAs could be implied in the pathogenesis of thromboembolic complications in coronavirus disease 2019 (COVID-19). We isolated circulating exosomes from patients with COVID-19, and then we divided our population in two arms based on the D-dimer level on hospital admission. We observed that exosomal miR-145 and miR-885 significantly correlate with D-dimer levels. Moreover, we demonstrate that human endothelial cells express the main cofactors needed for the internalization of the "Severe acute respiratory syndrome coronavirus 2" (SARS-CoV-2), including angiotensin converting enzyme 2, transmembrane protease serine 2, and CD-147. Interestingly, human endothelial cells treated with serum from COVID-19 patients release significantly less miR-145 and miR-885, exhibit increased apoptosis, and display significantly impaired angiogenetic properties compared with cells treated with non-COVID-19 serum. Taken together, our data indicate that exosomal miR-145 and miR-885 are essential in modulating thromboembolic events in COVID-19. SIGNIFICANCE STATEMENT: This work demonstrates for the first time that two specific microRNAs (namely miR-145 and miR-885) contained in circulating exosomes are functionally involved in thromboembolic events in COVID-19. These findings are especially relevant to the general audience when considering the emerging prominence of post-acute sequelae of COVID-19 systemic manifestations known as Long COVID.
Identifiants
pubmed: 35772782
pii: jpet.122.001209
doi: 10.1124/jpet.122.001209
pmc: PMC9827505
doi:
Substances chimiques
MicroRNAs
0
MIRN145 microRNA, human
0
MIRN885 microRNA, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
109-115Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK123259
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK033823
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL159062
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL146691
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL144456
Pays : United States
Informations de copyright
Copyright © 2022 by The American Society for Pharmacology and Experimental Therapeutics.
Références
Virus Res. 2020 Oct 15;288:198114
pubmed: 32800805
Cell Metab. 2021 Apr 6;33(4):781-790.e5
pubmed: 33450179
JCI Insight. 2021 Oct 22;6(20):
pubmed: 34506304
Biomedicines. 2021 Aug 05;9(8):
pubmed: 34440170
Am J Physiol Cell Physiol. 2022 Jan 1;322(1):C1-C11
pubmed: 34817268
Signal Transduct Target Ther. 2020 Dec 4;5(1):283
pubmed: 33277466
ESC Heart Fail. 2020 Feb;7(1):284-288
pubmed: 31981320
Clin Microbiol Infect. 2020 Nov;26(11):1448-1449
pubmed: 32712242
Int J Mol Sci. 2021 Aug 12;22(16):
pubmed: 34445368
Int J Cardiol. 2021 Mar 1;326:230-236
pubmed: 32979425
Noncoding RNA. 2021 Sep 22;7(4):
pubmed: 34698261
Vascul Pharmacol. 2022 Jun;144:106975
pubmed: 35248780
Lancet Respir Med. 2022 Feb;10(2):199-213
pubmed: 34767767
J Pharmacol Exp Ther. 2022 Jun;381(3):197-203
pubmed: 35339987
Oxid Med Cell Longev. 2018 Dec 19;2018:9582319
pubmed: 30662585
Antioxidants (Basel). 2021 Aug 25;10(9):
pubmed: 34572973
JAMA. 2022 Apr 5;327(13):1236-1246
pubmed: 35285853
Biology (Basel). 2022 Jan 02;11(1):
pubmed: 35053059
J Clin Med. 2022 Apr 15;11(8):
pubmed: 35456321
Crit Care. 2021 Nov 8;25(1):381
pubmed: 34749792
Nutrients. 2021 Nov 05;13(11):
pubmed: 34836206
Nat Commun. 2022 Apr 12;13(1):1957
pubmed: 35413949
BMJ Open. 2021 Jun 25;11(6):e047561
pubmed: 34172549
Biomedicines. 2020 Oct 30;8(11):
pubmed: 33143053
Expert Rev Hematol. 2022 Mar;15(3):243-251
pubmed: 35262442
J Infect. 2021 Mar;82(3):378-383
pubmed: 33450302
Am J Emerg Med. 2021 Feb;40:55-59
pubmed: 33348224
Methods Mol Biol. 2022;2455:333-341
pubmed: 35213005
Int J Mol Sci. 2019 Dec 27;21(1):
pubmed: 31892162
Eur Respir J. 2022 Aug 4;60(2):
pubmed: 35144991
Stroke Vasc Neurol. 2020 Sep;5(3):279-284
pubmed: 32616524
Int J Mol Sci. 2021 Dec 07;22(24):
pubmed: 34947989
Noncoding RNA. 2021 Aug 31;7(3):
pubmed: 34564316
J Clin Med. 2022 Mar 25;11(7):
pubmed: 35407430
J Thromb Haemost. 2020 Jun;18(6):1324-1329
pubmed: 32306492
J Biol Chem. 2022 Mar;298(3):101695
pubmed: 35143839
Biomedicines. 2020 Aug 06;8(8):
pubmed: 32781796
Mol Cancer. 2009 Nov 09;8:97
pubmed: 19900276
Eur Heart J. 2020 Sep 1;41(32):3038-3044
pubmed: 32882706
Noncoding RNA. 2021 Nov 29;7(4):
pubmed: 34940755
Front Mol Biosci. 2021 Feb 24;8:630718
pubmed: 33718435
J Clin Med. 2020 May 11;9(5):
pubmed: 32403217
EBioMedicine. 2017 Dec;26:175-186
pubmed: 29217135
J Clin Med. 2021 May 13;10(10):
pubmed: 34068397
Clin Appl Thromb Hemost. 2021 Jan-Dec;27:10760296211017668
pubmed: 34011194
Clin Lab. 2022 Feb 1;68(2):
pubmed: 35142198
PLoS One. 2021 Aug 26;16(8):e0256744
pubmed: 34437642
J Pharmacol Exp Ther. 2021 Nov;379(2):182-190
pubmed: 34389654
Am J Med Sci. 2022 Apr;363(4):281-287
pubmed: 35093394
J Clin Invest. 2022 Feb 15;132(4):
pubmed: 35166236
Front Cell Dev Biol. 2021 Jan 07;8:602493
pubmed: 33490068
Front Cell Dev Biol. 2022 Jan 18;9:771354
pubmed: 35118066
BMJ Glob Health. 2021 Sep;6(9):
pubmed: 34580069
Life (Basel). 2021 Jun 14;11(6):
pubmed: 34198591
Handb Exp Pharmacol. 2006;(176 Pt 2):285-306
pubmed: 16999230
Annu Rev Med. 1996;47:315-31
pubmed: 8712785
Cells. 2022 Mar 09;11(6):
pubmed: 35326383
Lancet Digit Health. 2022 Apr;4(4):e220-e234
pubmed: 35337642
BMJ. 2022 Apr 4;377:o892
pubmed: 35379691
Clin Exp Immunol. 2020 Nov;202(2):162-192
pubmed: 32935331
Redox Biol. 2021 Nov 6;48:102181
pubmed: 34768063
Nat Rev Immunol. 2020 Jul;20(7):389-391
pubmed: 32439870
EClinicalMedicine. 2021 May 24;36:100899
pubmed: 34036253
J Cell Physiol. 2022 Apr;237(4):2078-2094
pubmed: 35137416
PLoS One. 2021 Jan 28;16(1):e0246030
pubmed: 33507974
J Clin Epidemiol. 2022 Feb;142:54-59
pubmed: 34715312
PLoS Pathog. 2021 Jul 28;17(7):e1009759
pubmed: 34320031
Emerg Radiol. 2021 Apr;28(2):423-429
pubmed: 33159219
J Am Coll Cardiol. 2020 Jun 16;75(23):2950-2973
pubmed: 32311448
Nat Rev Cardiol. 2021 Mar;18(3):194-209
pubmed: 33214651
Oncol Rep. 2019 Jan;41(1):279-291
pubmed: 30542696
Front Med (Lausanne). 2022 Mar 22;9:834354
pubmed: 35391879
Expert Rev Hematol. 2020 Nov;13(11):1265-1275
pubmed: 32997543
EClinicalMedicine. 2021 Oct;40:101125
pubmed: 34522871
Noncoding RNA. 2021 Feb 02;7(1):
pubmed: 33540664
Eur Heart J Cardiovasc Pharmacother. 2021 May 23;7(3):e2-e3
pubmed: 33377481
BMJ Open. 2022 Feb 7;12(2):e057408
pubmed: 35131836
Scott Med J. 2020 Nov;65(4):127-132
pubmed: 32807018
J Thromb Haemost. 2022 Apr;20(4):1008-1014
pubmed: 35102689
Sci Rep. 2021 Jun 10;11(1):12298
pubmed: 34112884
Lancet Respir Med. 2022 Feb;10(2):214-220
pubmed: 34838161
Aging (Albany NY). 2021 Jan 10;13(3):4079-4095
pubmed: 33461167
J Transl Med. 2011 Jan 13;9:7
pubmed: 21232121
Clin Appl Thromb Hemost. 2022 Jan-Dec;28:10760296221074353
pubmed: 35068227
Noncoding RNA. 2021 Jun 18;7(2):
pubmed: 34207055
Cell Signal. 2021 Jan;77:109812
pubmed: 33164880
BMJ. 2021 Jul 26;374:n1648
pubmed: 34312178
Cells. 2020 Jul 06;9(7):
pubmed: 32640511
Front Cell Dev Biol. 2022 Feb 15;10:824851
pubmed: 35242762
Eur J Pharmacol. 2020 Dec 15;889:173644
pubmed: 33053381
Lancet Reg Health Eur. 2022 Jun;17:100363
pubmed: 35434687
Microcirculation. 2021 Oct;28(7):e12718
pubmed: 34013612
Crit Care. 2021 Aug 25;25(1):306
pubmed: 34433473
J Biol Chem. 2021 Jul;297(1):100847
pubmed: 34058196
Front Immunol. 2020 Oct 07;11:576745
pubmed: 33117379
Front Immunol. 2022 Jan 14;12:799558
pubmed: 35095880
Int J Dermatol. 2021 Feb;60(2):229-232
pubmed: 33259066
Theranostics. 2021 Jul 6;11(16):8076-8091
pubmed: 34335981
Int Immunopharmacol. 2021 Nov;100:108071
pubmed: 34482267