Infection susceptibility and vector competence of Rhodnius robustus Larrousse, 1927 and R. pictipes Stal, 1872 (Hemiptera, Reduviidae, Triatominae) for strains of Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) I, II and IV.
Chagas disease
DTUs
Experimental infection
Triatomines
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
30 Jun 2022
30 Jun 2022
Historique:
received:
15
03
2022
accepted:
31
05
2022
entrez:
30
6
2022
pubmed:
1
7
2022
medline:
6
7
2022
Statut:
epublish
Résumé
Rhodnius robustus and Rhodnius pictipes are vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease (CD), that are found in the Brazilian Amazon region. Susceptibility to infection and vector competence depend on the parasite-vector relationship. Our objective was to evaluate the interaction between T. cruzi and these two triatomine vectors in pure and mixed experimental infections of T. cruzi strains from the same or different geographic regions. Fifth-instar nymphs of R. robustus and R. pictipes were fed on mice infected with four T. cruzi strains, namely genotypes TcIAM, TcIMG, TcIIPR, and TcIVAM, respectively, from the Brazilian states of Amazonas, Minas Gerais and Paraná. Over a period of 120 days, excreta were examined every 20 days to assess vector competence, and intestinal contents (IC) were examined every 30 days to determine susceptibility to infection. The highest positive rate in the fresh examination (%+FE, 30.0%), the highest number of parasitic forms (PF, n = 1969) and the highest metacyclogenesis rate (%MC, 53.8%) in the excreta were recorded for R. robustus/TcIVAM. Examination of the IC of R. pictipes revealed a higher number of PF in infections with TcIAM (22,680 PF) and TcIIPR (19,845 PF) alone or in association (17,145 PF), as well as a %+FE of 75.0% with TcII, in comparison with the other genotypes. The highest %MC (100%) was recorded for the mixed infections of TcIAM with TcIIPR or TcIVAM in the IC of R. pictipes. Overall, both species were found to be susceptible to the T. cruzi strains studied. Rhodnius robustus showed vector competence for genotypes TcIVAM and TcIAM+TcIVAM and R. pictipes for TcIAM+TcIVAM and TcIAM+TcIIPR; there was elimination of infective forms as early as at 20 days. Our results suggest that both the genetics of the parasite and its geographic origin influence the susceptibility to infection and vector competence, alone or in association.
Sections du résumé
BACKGROUND
BACKGROUND
Rhodnius robustus and Rhodnius pictipes are vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease (CD), that are found in the Brazilian Amazon region. Susceptibility to infection and vector competence depend on the parasite-vector relationship. Our objective was to evaluate the interaction between T. cruzi and these two triatomine vectors in pure and mixed experimental infections of T. cruzi strains from the same or different geographic regions.
METHODS
METHODS
Fifth-instar nymphs of R. robustus and R. pictipes were fed on mice infected with four T. cruzi strains, namely genotypes TcIAM, TcIMG, TcIIPR, and TcIVAM, respectively, from the Brazilian states of Amazonas, Minas Gerais and Paraná. Over a period of 120 days, excreta were examined every 20 days to assess vector competence, and intestinal contents (IC) were examined every 30 days to determine susceptibility to infection.
RESULTS
RESULTS
The highest positive rate in the fresh examination (%+FE, 30.0%), the highest number of parasitic forms (PF, n = 1969) and the highest metacyclogenesis rate (%MC, 53.8%) in the excreta were recorded for R. robustus/TcIVAM. Examination of the IC of R. pictipes revealed a higher number of PF in infections with TcIAM (22,680 PF) and TcIIPR (19,845 PF) alone or in association (17,145 PF), as well as a %+FE of 75.0% with TcII, in comparison with the other genotypes. The highest %MC (100%) was recorded for the mixed infections of TcIAM with TcIIPR or TcIVAM in the IC of R. pictipes.
CONCLUSIONS
CONCLUSIONS
Overall, both species were found to be susceptible to the T. cruzi strains studied. Rhodnius robustus showed vector competence for genotypes TcIVAM and TcIAM+TcIVAM and R. pictipes for TcIAM+TcIVAM and TcIAM+TcIIPR; there was elimination of infective forms as early as at 20 days. Our results suggest that both the genetics of the parasite and its geographic origin influence the susceptibility to infection and vector competence, alone or in association.
Identifiants
pubmed: 35773725
doi: 10.1186/s13071-022-05350-3
pii: 10.1186/s13071-022-05350-3
pmc: PMC9245265
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
239Subventions
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná
ID : 10943812,251//2014
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 483469/2013-0
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financing Code 001
Informations de copyright
© 2022. The Author(s).
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