Metabarcoding of Fish Larvae in the Merbok River Reveals Species Diversity and Distribution Along its Mangrove Environment.
DNA metabarcoding
Fish larvae
Mangrove estuary
Merbok River
Next-generation sequencing
Journal
Zoological studies
ISSN: 1810-522X
Titre abrégé: Zool Stud
Pays: China (Republic : 1949- )
ID NLM: 9889708
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
11
2020
accepted:
11
10
2021
entrez:
1
7
2022
pubmed:
2
7
2022
medline:
2
7
2022
Statut:
epublish
Résumé
The Merbok River (north-west of Peninsular Malaysia) is a mangrove estuary that provides habitat for over 100 species of fish, which are economically and ecologically important. Threats such as habitat loss and overfishing are becoming a great concern for fisheries conservation and management. The identification of larval fish in this estuarine system is important to complement information on the adults. This is because the data could inform the spawning behaviour, reproductive biology, selection of nursery grounds and migration route of fish. Such information is invaluable for fisheries and aquatic environmental monitoring, and thus for their conservation and management. However, identifying fish larvae is a challenging task based only on morphology and even traditional DNA barcoding. To address this, DNA metabarcoding was utilised to detect the diversity of fish in the Merbok River. To complete the study, the fish larvae were collected at six sampling sites of the river. The extracted larval DNA was amplified for the Cytochrome Oxidase subunit 1 (
Identifiants
pubmed: 35774258
doi: 10.6620/ZS.2021.60-76
pmc: PMC9169113
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e76Références
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