Human NK cells responses are enhanced by CD56 engagement.
CD56
activating receptors
cytokines
degranulation
natural killer cells
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
03
06
2022
received:
23
02
2022
accepted:
29
06
2022
pubmed:
2
7
2022
medline:
9
9
2022
entrez:
1
7
2022
Statut:
ppublish
Résumé
Natural Killer (NK) cells are important innate lymphocytes for effective immune responses against intracellular pathogens and tumors. CD56 is a well-known marker for human NK cells, but there is very limited information about a functional role of this surface receptor. Here, we show that engagement of CD56 can induce NK cell activation resulting in degranulation, IFN-γ secretion and morphological changes, making CD56 a potential co-activating receptor in NK cells. Interestingly, this effect was only observed in cytokine pre-activated and not in freshly isolated human NK cells, demonstrating that NK cell reactivity upon CD56 engagement was dependent on cytokine stimulation. Inhibition of Syk, PI3K, Erk, and src-family-kinases impaired CD56-mediated NK cell stimulation. Finally, we used CRISPR/Cas9 to delete CD56 from primary human NK cells. While this abolished the stimulatory effect of CD56 on pre-activated NK cells, the cytotoxic activity of NK cells against several tumor target cells was not affected by the absence of CD56. This demonstrates that the stimulating effect of CD56 on pre-activated NK cells does not have a major impact on their cytotoxic activity, but it may contribute to the function of CD56 as a fungal recognition receptor and in the NK cell developmental synapse.
Identifiants
pubmed: 35775327
doi: 10.1002/eji.202249868
doi:
Substances chimiques
CD56 Antigen
0
Cytokines
0
NCAM1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1441-1451Informations de copyright
© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.
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