Impact of Inflammation on Midazolam Metabolism in Severe COVID-19 Patients.


Journal

Clinical pharmacology and therapeutics
ISSN: 1532-6535
Titre abrégé: Clin Pharmacol Ther
Pays: United States
ID NLM: 0372741

Informations de publication

Date de publication:
11 2022
Historique:
received: 29 11 2021
accepted: 12 06 2022
pubmed: 2 7 2022
medline: 25 10 2022
entrez: 1 7 2022
Statut: ppublish

Résumé

Midazolam is a benzodiazepine frequently used for sedation in patients hospitalized in the intensive care unit (ICU) for coronavirus disease 2019 (COVID-19). This drug is primarily metabolized by cytochrome P450 3A (CYP3A) isoenzymes. Several studies have suggested that inflammation, frequently observed in these patients, could modulate CYP3A activity. The objective of this work was to study the impact of inflammation on midazolam pharmacokinetics in patients with COVID-19. Forty-eight patients hospitalized in the ICU for COVID-19 and treated with midazolam administered by continuous infusion were included in this study. Midazolam and α-hydroxymidazolam concentrations were measured and patient data, including the use of CYP3A inhibitors, were collected. Total and unbound concentrations of midazolam and α-hydroxymidazolam were measured in plasma using a validated liquid-chromatography coupled with mass spectrometry method. Inflammatory condition was evaluated by C-reactive protein (CRP) level measurement. Both drug concentrations and CRP measurements were performed on 354 plasma samples. CRP elevation was significantly associated with the α-hydroxymidazolam/midazolam plasma ratio decrease, whether for the unbound fraction or for the total fraction. Conversely, inflammation was not associated with protein binding modifications. Logically, α-hydroxymidazolam/midazolam plasma ratio was significantly reduced when patients were treated with CYP3A inhibitors. In this study, we showed that inflammation probably reduces the metabolism of midazolam by CYP3A. These results suggest that molecules with narrow therapeutic margins and metabolized by CYP3A should be administrated with care in case of massive inflammatory situations.

Identifiants

pubmed: 35776074
doi: 10.1002/cpt.2698
pmc: PMC9350233
doi:

Substances chimiques

Midazolam R60L0SM5BC
Cytochrome P-450 CYP3A EC 1.14.14.1
Isoenzymes 0
C-Reactive Protein 9007-41-4
Cytochrome P-450 CYP3A Inhibitors 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1033-1039

Commentaires et corrections

Type : CommentIn
Type : CommentIn

Informations de copyright

© 2022 The Authors. Clinical Pharmacology & Therapeutics © 2022 American Society for Clinical Pharmacology and Therapeutics.

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Auteurs

Edouard Charles Le Carpentier (EC)

Laboratoire de Pharmacologie Clinique, CHU Nantes, Nantes Université, Nantes, France.

Emmanuel Canet (E)

Médecine Intensive Réanimation, CHU Nantes, Nantes Université, Nantes, France.

Damien Masson (D)

Laboratoire de Biochimie, CHU Nantes, Nantes Université, Nantes, France.

Maëlle Martin (M)

Médecine Intensive Réanimation, CHU Nantes, Nantes Université, Nantes, France.

Guillaume Deslandes (G)

Laboratoire de Pharmacologie Clinique, CHU Nantes, Nantes Université, Nantes, France.

Aurélie Gaultier (A)

Plateforme de Méthodologie et Biostatistique, CHU Nantes, Nantes Université, Nantes, France.

Éric Dailly (É)

Laboratoire de Pharmacologie Clinique, CHU Nantes, Nantes Université, Nantes, France.
Cibles et Médicaments des Infections et de l'Immunité, IICiMed, CHU Nantes, Nantes Université, Nantes, France.

Ronan Bellouard (R)

Laboratoire de Pharmacologie Clinique, CHU Nantes, Nantes Université, Nantes, France.
Cibles et Médicaments des Infections et de l'Immunité, IICiMed, CHU Nantes, Nantes Université, Nantes, France.

Matthieu Gregoire (M)

Laboratoire de Pharmacologie Clinique, CHU Nantes, Nantes Université, Nantes, France.
The Enteric Nervous System in Gut and Brain Disorders, IMAD, INSERM, CHU Nantes, Nantes Université, Nantes, France.

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