Membrane Binding Induces Distinct Structural Signatures in the Mouse Complexin-1C-Terminal Domain.
Curvature sensitivity
Disorder-to-order transition
Protein:vesicle interactions
SNARE-mediated exocytosis regulation
Synaptic vesicle trafficking
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
15 01 2023
15 01 2023
Historique:
received:
10
05
2022
revised:
24
06
2022
accepted:
24
06
2022
pmc-release:
15
01
2024
pubmed:
2
7
2022
medline:
4
1
2023
entrez:
1
7
2022
Statut:
ppublish
Résumé
Complexins play a critical role in regulating SNARE-mediated exocytosis of synaptic vesicles. Evolutionary divergences in complexin function have complicated our understanding of the role these proteins play in inhibiting the spontaneous fusion of vesicles. Previous structural and functional characterizations of worm and mouse complexins have indicated the membrane curvature-sensing C-terminal domain of these proteins is responsible for differences in inhibitory function. We have characterized the structure and dynamics of the mCpx1 CTD in the absence and presence of membranes and membrane mimetics using NMR, ESR, and optical spectroscopies. In the absence of lipids, the mCpx1 CTD features a short helix near its N-terminus and is otherwise disordered. In the presence of micelles and small unilamellar vesicles, the mCpx1 CTD forms a discontinuous helical structure in its C-terminal 20 amino acids, with no preference for specific lipid compositions. In contrast, the mCpx1 CTD shows distinct compositional preferences in its interactions with large unilamellar vesicles. These studies identify structural divergences in the mCpx1 CTD relative to the wCpx1 CTD in regions that are known to be critical to the wCpx1 CTD's role in inhibiting spontaneous fusion of synaptic vesicles, suggesting a potential structural basis for evolutionary divergences in complexin function.
Identifiants
pubmed: 35777466
pii: S0022-2836(22)00302-3
doi: 10.1016/j.jmb.2022.167710
pmc: PMC9794636
mid: NIHMS1823731
pii:
doi:
Substances chimiques
Adaptor Proteins, Vesicular Transport
0
Calcium
SY7Q814VUP
Nerve Tissue Proteins
0
SNARE Proteins
0
Unilamellar Liposomes
0
complexin I
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
167710Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM136686
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123779
Pays : United States
Organisme : NIH HHS
ID : S10 OD016432
Pays : United States
Organisme : NIGMS NIH HHS
ID : RM1 GM145397
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM117518
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM118302
Pays : United States
Organisme : NIH HHS
ID : S10 OD018509
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103521
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM066354
Pays : United States
Organisme : NCRR NIH HHS
ID : C06 RR015495
Pays : United States
Organisme : NIH HHS
ID : S10 OD021543
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Références
Neurosci Lett. 1997 Aug 29;232(2):107-10
pubmed: 9302098
Mol Cell Neurosci. 2013 Jan;52:161-72
pubmed: 23159779
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20777-82
pubmed: 24297916
J Biomol NMR. 2013 Jul;56(3):227-41
pubmed: 23728592
Pflugers Arch. 2020 Jan;472(1):117-133
pubmed: 31875236
Eur J Hum Genet. 2017 Jun;25(7):889-893
pubmed: 28422131
Biophys J. 2016 Nov 1;111(9):1954-1964
pubmed: 27806277
Mol Cell Neurosci. 2010 Dec;45(4):389-97
pubmed: 20678575
J Biol Chem. 2002 Jul 19;277(29):26517-23
pubmed: 12004067
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):E4698-707
pubmed: 27444020
Nat Neurosci. 2015 Jul;18(7):935-41
pubmed: 26108721
FEBS Lett. 2009 Jul 21;583(14):2343-8
pubmed: 19540234
Bioinformatics. 2015 Apr 15;31(8):1325-7
pubmed: 25505092
Cell. 2001 Jan 12;104(1):71-81
pubmed: 11163241
Nat Struct Mol Biol. 2022 Feb;29(2):97-107
pubmed: 35132256
Biochem Biophys Res Commun. 1995 Aug 24;213(3):1107-14
pubmed: 7654227
Elife. 2022 Apr 20;11:
pubmed: 35442188
Methods Enzymol. 2011;492:189-211
pubmed: 21333792
Elife. 2014 May 08;3:e02391
pubmed: 24842998
Nat Struct Mol Biol. 2008 Jul;15(7):665-74
pubmed: 18618940
Methods Mol Biol. 2020;2141:555-567
pubmed: 32696377
Front Mol Neurosci. 2017 May 26;10:146
pubmed: 28603484
Curr Biol. 2011 Jan 25;21(2):106-13
pubmed: 21215631
Elife. 2014 Nov 10;3:
pubmed: 25383924
Traffic. 2008 Sep;9(9):1403-13
pubmed: 18445121
J Am Chem Soc. 2001 Apr 4;123(13):2970-8
pubmed: 11457007
Neuron. 2012 Jul 12;75(1):11-25
pubmed: 22794257
Neuron. 2013 Oct 30;80(3):675-90
pubmed: 24183019
Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16
pubmed: 23962882
Nat Neurosci. 2007 Oct;10(10):1235-7
pubmed: 17873870
Neuron. 2002 Jan 31;33(3):397-409
pubmed: 11832227
Cold Spring Harb Perspect Biol. 2011 Dec 01;3(12):
pubmed: 22026965
Nat Commun. 2014 Sep 17;5:4955
pubmed: 25229806
Curr Biol. 2011 Jan 25;21(2):97-105
pubmed: 21215634
Biophys J. 2017 Sep 19;113(6):1235-1250
pubmed: 28456331
Cell. 1995 Oct 6;83(1):111-9
pubmed: 7553862
Elife. 2016 Jun 02;5:
pubmed: 27253060
Biochem Biophys Res Commun. 2007 Mar 16;354(3):808-13
pubmed: 17266930
Annu Rev Biophys. 2015;44:339-67
pubmed: 26098518
Neuron. 2015 Nov 18;88(4):749-61
pubmed: 26590346
J Cell Biol. 2005 May 23;169(4):669-80
pubmed: 15911881
J Biomol NMR. 1995 Nov;6(3):277-93
pubmed: 8520220
Hum Mol Genet. 2005 Aug 15;14(16):2369-85
pubmed: 16000319
J Phys Chem A. 2019 Jan 10;123(1):359-370
pubmed: 30525624
Mol Cell Neurosci. 2013 Sep;56:244-54
pubmed: 23769723
J Biomol NMR. 2011 Jun;50(2):157-65
pubmed: 21604143
J Biol Chem. 2000 Jun 30;275(26):19808-18
pubmed: 10777504
Neuron. 2013 Jan 23;77(2):323-34
pubmed: 23352168
J Neurosci. 2012 Feb 22;32(8):2877-85
pubmed: 22357870
Neuron. 2009 Nov 12;64(3):367-80
pubmed: 19914185
Nat Struct Mol Biol. 2007 Oct;14(10):949-58
pubmed: 17828276
Biophys J. 2017 Apr 25;112(8):1529-1534
pubmed: 28445744
eNeuro. 2021 Apr 7;8(2):
pubmed: 33692090
J Biomol NMR. 2012 Oct;54(2):181-91
pubmed: 22960996
J Biomol NMR. 2011 Feb;49(2):139-49
pubmed: 21234644
Front Mol Neurosci. 2017 May 24;10:154
pubmed: 28596722
Neuron. 2015 Nov 4;88(3):499-513
pubmed: 26539891
Methods Enzymol. 2015;564:349-87
pubmed: 26477257
Nat Rev Neurosci. 2016 Feb;17(2):118-25
pubmed: 26806630
PLoS Biol. 2018 Apr 9;16(4):e2003611
pubmed: 29630591
Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):E7590-E7599
pubmed: 27821736
Nat Struct Mol Biol. 2011 Jul 24;18(8):927-33
pubmed: 21785414
J Mol Biol. 2009 Jul 24;390(4):775-90
pubmed: 19481095
Science. 2009 Jan 23;323(5913):516-21
pubmed: 19164751