Molecular detection of haemophilic pathogens reveals evidence of Candidatus Mycoplasma haemobos in dogs and parasitic ticks in central China.
Dog
Haemaphysalis longicornis
Rhipicephalus (Boophilus) microplus
Transmission
‘Candidatus Mycoplasma haemobos’
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
01 Jul 2022
01 Jul 2022
Historique:
received:
18
04
2022
accepted:
22
06
2022
entrez:
1
7
2022
pubmed:
2
7
2022
medline:
7
7
2022
Statut:
epublish
Résumé
In addition to Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum, a few hemoplasma species that mainly infect other livestock have been detected in dogs. 'Candidatus Mycoplasma haemobos' (Ca. M. haemobos) has been found in a variety of animals in China. The present study was aimed to investigate the occurrence of 'Ca. M. haemobos' infections in dogs and ticks collected from the Henan province, China. Overall, 55 dog blood samples and 378 ticks on skins were collected from anemic and healthy dogs, and these samples were subjected to PCR, sequence analysis, and identification. The results showed that Haemaphysalis longicornis (266) and Rhipicephalus (Boophilus) microplus (112) were the only two parasitic ticks on dogs. Molecular detection revealed that 163 M. haemocanis, 88 'Ca. M. haemobos' and 32 Anaplasma platys positive amplicons could be amplified from dogs, H. longicornis and R. (B.) microplus. In addition, co-infections (M. haemocanis + A. platys and 'Ca. M. haemobos'+ A. platys) could be also detected. To the best of our knowledge, this is the first molecular evidence of 'Ca. M. haemobos' natural infection in dogs and tick species identified as H. longicornis and R. (B.) microplus from China.
Sections du résumé
BACKGROUND
BACKGROUND
In addition to Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum, a few hemoplasma species that mainly infect other livestock have been detected in dogs. 'Candidatus Mycoplasma haemobos' (Ca. M. haemobos) has been found in a variety of animals in China. The present study was aimed to investigate the occurrence of 'Ca. M. haemobos' infections in dogs and ticks collected from the Henan province, China.
RESULTS
RESULTS
Overall, 55 dog blood samples and 378 ticks on skins were collected from anemic and healthy dogs, and these samples were subjected to PCR, sequence analysis, and identification. The results showed that Haemaphysalis longicornis (266) and Rhipicephalus (Boophilus) microplus (112) were the only two parasitic ticks on dogs. Molecular detection revealed that 163 M. haemocanis, 88 'Ca. M. haemobos' and 32 Anaplasma platys positive amplicons could be amplified from dogs, H. longicornis and R. (B.) microplus. In addition, co-infections (M. haemocanis + A. platys and 'Ca. M. haemobos'+ A. platys) could be also detected.
CONCLUSIONS
CONCLUSIONS
To the best of our knowledge, this is the first molecular evidence of 'Ca. M. haemobos' natural infection in dogs and tick species identified as H. longicornis and R. (B.) microplus from China.
Identifiants
pubmed: 35778709
doi: 10.1186/s12917-022-03361-x
pii: 10.1186/s12917-022-03361-x
pmc: PMC9248110
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
254Subventions
Organisme : National Natural Science Foundation of China
ID : 31902263
Organisme : National Natural Science Foundation of China
ID : 31870917
Organisme : Scientific and Technological Project of Henan Province
ID : 222102110438
Organisme : Nanyang Normal University
ID : 15081
Organisme : program for Innovative Research Team of Science and Technology in University of Henan Province
ID : 20IRTSTHN024
Informations de copyright
© 2022. The Author(s).
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