The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro.


Journal

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295

Informations de publication

Date de publication:
Sep 2022
Historique:
received: 24 05 2022
revised: 24 06 2022
accepted: 24 06 2022
pubmed: 3 7 2022
medline: 9 9 2022
entrez: 2 7 2022
Statut: ppublish

Résumé

In previous work, we tested the immunomodulatory effect of Nigella sativa (NS) fatty oil. Our results demonstrated that unrefined, obtained by cold pressing black cumin seed oil inhibited lymphocytes' proliferation and induced their apoptosis in a dose-dependent manner. In this study, we examined the immunomodulatory properties of essential oil (EO) obtained from the NS seeds by hydrodistillation and its two main constituents: thymoquinone (TQ) and p-cymene. We analyzed the proliferation, activation phenotype, and apoptosis rates of human T lymphocytes stimulated with an immobilized monoclonal anti-CD3 antibody in the presence of serial ethanol dilutions of tested oil or serial distilled water dilutions of tested compounds with flow cytometry. Our results showed that NSEO significantly inhibited the proliferation of CD4

Identifiants

pubmed: 35779419
pii: S0753-3322(22)00738-7
doi: 10.1016/j.biopha.2022.113349
pii:
doi:

Substances chimiques

Benzoquinones 0
Oils, Volatile 0
Plant Oils 0
caraway oil C2J9B08Q3I

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

113349

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest statement The authors declare no conflict of interest.

Auteurs

Klaudia Ciesielska-Figlon (K)

Department of Physiopathology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.

Agnieszka Daca (A)

Division of Pathology and Experimental Rheumatology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.

Adam Kokotkiewicz (A)

Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdańsk, Poland.

Maria Łuczkiewicz (M)

Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdańsk, Poland.

Bożena Zabiegała (B)

Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, Gdańsk, Poland.

Jacek M Witkowski (JM)

Department of Physiopathology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland.

Katarzyna A Lisowska (KA)

Department of Physiopathology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland. Electronic address: katarzyna.lisowska@gumed.edu.pl.

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Classifications MeSH