Genomic Characteristics of a Chicken Infectious Anemia Virus in Contaminated Attenuated Vaccine.
chicken infectious anemia virus
contamination
genome analysis
live vaccines
recombinase-aided amplification assay
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2022
2022
Historique:
received:
22
04
2022
accepted:
25
05
2022
entrez:
5
7
2022
pubmed:
6
7
2022
medline:
6
7
2022
Statut:
epublish
Résumé
Chicken infectious anemia virus (CIAV) can be transmitted by contaminated live vaccines, and causes huge economic losses. This study evaluated the contamination status of CIAV in 24 batches of vaccines by recombinase-aided amplification assay (RAA), fluorescence quantitative PCR and dot blot assay, and then found a contaminated attenuated vaccine. The whole genome of the CIAV contaminant was then sequenced and named JS2020-PFV (Genbank accession number: MW234428, 2296bp). It showed 94.5 to 99.9% identities with reference strains and shared the closest evolution relationship with AB1K strain which was isolated from a chicken farm in Turkey. All of these suggested that the use of CIAV contaminated live vaccine may be one of the reason for its epidemic in poultry.
Identifiants
pubmed: 35782569
doi: 10.3389/fvets.2022.925935
pmc: PMC9244546
doi:
Types de publication
Journal Article
Langues
eng
Pagination
925935Informations de copyright
Copyright © 2022 Chen, Su, Li, Wang, Zhang, Chang, Wang and Zhao.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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