Thicker Ice Improves the Integrity and Angular Distribution of CDC48A Hexamers on Cryo-EM Grids.
cryo-electron microscopy
grid preparation
ice thickness
oligomer disassembly
optimization
particle integrity
preferential orientation
single particle analysis
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2022
2022
Historique:
received:
05
03
2022
accepted:
26
05
2022
entrez:
5
7
2022
pubmed:
6
7
2022
medline:
6
7
2022
Statut:
epublish
Résumé
Many cryogenic electron microscopy (cryo-EM) single particle analyses are constrained by the sample preparation step upon which aggregation, dissociation, and/or preferential orientation of particles can be introduced. Here, we report how we solved these problems in the case of CDC48A, a hexameric AAA ATPase from
Identifiants
pubmed: 35782862
doi: 10.3389/fmolb.2022.890390
pii: 890390
pmc: PMC9247313
doi:
Types de publication
Journal Article
Langues
eng
Pagination
890390Informations de copyright
Copyright © 2022 Huntington, Zhao, Bron, Shahul Hameed, Arold and Qureshi.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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