An Undergraduate Laboratory Exploring Mutational Mechanisms in Escherichia coli Based on the Luria-Delbrück Experiment.
Escherichia coli
Luria-Delbrück
antibiotic resistance
genetics
mismatch repair
mutation
Journal
Journal of microbiology & biology education
ISSN: 1935-7877
Titre abrégé: J Microbiol Biol Educ
Pays: United States
ID NLM: 101543341
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
03
08
2021
accepted:
06
02
2022
entrez:
5
7
2022
pubmed:
6
7
2022
medline:
6
7
2022
Statut:
epublish
Résumé
Understanding the mechanism for DNA mutations is a key concept in most genetics and microbiology courses. In addition, understanding that most mutations occur prior to exposure to selection is an important yet often difficult concept for students to grasp. We developed an undergraduate laboratory activity on mutation mechanisms based on the classic experiment from Luria and Delbrück. The activity uses Escherichia coli as the model organism and the antibiotic streptomycin for selection. Students gain hands-on experience with an important experiment in genetics, and the laboratory contains an investigative component in having students calculate mutation rate for streptomycin resistance and in having the students design a follow-up experiment. E. coli has a knockout collection available, and we used a wild-type strain and a Δ
Identifiants
pubmed: 35784616
doi: 10.1128/jmbe.00211-21
pii: jmbe00211-21
pmc: PMC9249129
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2022 Hutchison et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Mol Gen Genet. 1992 Mar;232(1):89-96
pubmed: 1552908
Appl Microbiol. 1970 Sep;20(3):447-51
pubmed: 5485725
Mol Syst Biol. 2006;2:2006.0008
pubmed: 16738554
Genetics. 1943 Nov;28(6):491-511
pubmed: 17247100
Genetics. 2012 May;191(1):21-32
pubmed: 22367036
Genetics. 2016 Feb;202(2):371-5
pubmed: 26869481
J Biol Chem. 2015 Oct 30;290(44):26395-403
pubmed: 26354434