Application of the Method of Melanin Bleaching After Immunohistochemical Staining of Melanin-containing Tissues.
Journal
Applied immunohistochemistry & molecular morphology : AIMM
ISSN: 1533-4058
Titre abrégé: Appl Immunohistochem Mol Morphol
Pays: United States
ID NLM: 100888796
Informations de publication
Date de publication:
01 08 2022
01 08 2022
Historique:
received:
22
01
2022
accepted:
23
05
2022
pubmed:
6
7
2022
medline:
9
8
2022
entrez:
5
7
2022
Statut:
ppublish
Résumé
The purpose of this research was to investigate the method of melanin bleaching in immunohistochemical staining of melanin-containing tissues. Forty melanin-containing tissue samples were selected, including 30 with malignant melanoma and 10 with blue naevi. The samples were divided into the following 3 groups: the prestaining bleaching group, nonbleaching group, and poststaining bleaching group. Each group was subjected to HMB45 and MelanA immunohistochemical staining. The detection system used was Roche ventana ultraview universal alkaline phosphatase red kit. In the prestaining bleaching group, melanin pigment was mostly removed. However, antigen expression was also affected, which resulted in significantly weaker positive staining and even represented false-negative expression. In the nonbleaching group, positive staining resulted in a red color that could easily be distinguished from the brown granules of melanin pigment. However, some melanin granules covered the tissue, impacting our observation of tissue structure. In the poststaining bleaching group, the results of positive staining were comparable to those in the nonbleaching group. The melanin pigment in these sections was almost completely removed, resulting in clearer staining and easier observation of tissue and cell structure. The method of melanin bleaching after immunohistochemical staining of melanin-containing tissue can almost completely bleach the melanin pigment and do not impact the expression of antigen. In our work, poststaining bleaching allowed for clearer immunohistochemical staining results and more obvious observation of tissue structure. This method also has the advantages of requiring simple reagents, utilizing simple operation procedures, and producing practical staining results, which makes it worthy of promotion!
Identifiants
pubmed: 35787527
doi: 10.1097/PAI.0000000000001047
pii: 00129039-202208000-00007
doi:
Substances chimiques
Melanins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
526-530Informations de copyright
Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Lapierre LM, Uckenpahler AL, Link BA, et al. Imaging melanin distribution in the zebrafish retina using photothermal optical coherence tomography. Transl Vis Sci Technol. 2018;7:4.
Wang CY, Wang JJ, Wang Y. Traditional depigmentation and immunohistochemistry of nevus tissue. J Clin Exp Pathol. 2018;34:228–229.
Wu H, Yuan J, Yan H, et al. Modified potassium permanganate. Application of oxalic acid depigmentation method in hematoxylin eosin staining and immunohistochemical detection of melanoma. Chin J Histochem Cytochem. 2017;26:510–513.
Manicam C, Pitz S, Brochhausen C, et al. Effective melanin depigmentation of human and murine ocular tissues: an improved method for paraffin and frozen sections. PLoS One. 2014;9:e102512.
Liang Y, Ling Q, Zhang W. Clinicopathological techniques. Beijing: People’s Health Publishing House; 2011:163.
Guo Y, Zhang M, Meng J, et al. An improved method for rapid removal of melanin from tissues. J Clin Exp Pathol. 2011;27:214.
Liu C-H, Lin C-H, Tsai M-J, et al. Melanin bleaching with dilute hydrogen peroxide: a simple and rapid method. Appl Immunohistochem Mol Morphol. 2013;21:275–279.
Hu L, Gao Y, Ren C, et al. Retaining antigenicity and DNA in the melanin bleaching of melanin-containing tissues. Int J Clin Exp Pathol. 2020;13:2027–2034.
Sun L, Huang B. Application of double oxidation depigmentation method in immunohistochemical diagnosis of malignant melanoma. Chin J Cancer Prev Treat. 2012;19:1576–1579.
Chen Q, Xia C, Wang M, et al. Application of modified hydrogen peroxide heating depigmentation method in immunohistochemical detection of melanoma. Chin J Pathol. 2014;43:482–484.
Shen H, Wu W. Study of melanin bleaching after immunohistochemistry of melanin-containing tissues. Appl Immunohistochem Mol Morphol. 2015;23:303–307.