Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
05 07 2022
05 07 2022
Historique:
received:
08
04
2021
accepted:
09
06
2022
entrez:
5
7
2022
pubmed:
6
7
2022
medline:
8
7
2022
Statut:
epublish
Résumé
Mammalian pre-implantation embryos accumulate substantial lipids, which are stored in lipid droplets (LDs). Despite the fundamental roles of lipids in many cellular functions, the significance of building-up LDs for the developing embryo remains unclear. Here we report that the accumulation and mobilization of LDs upon implantation are causal in the morphogenesis of the pluripotent epiblast and generation of the pro-amniotic cavity in mouse embryos, a critical step for all subsequent development. We show that the CIDEA protein, found abundantly in adipocytes, enhances lipid storage in blastocysts and pluripotent stem cells by promoting LD enlargement through fusion. The LD-stored lipids are mobilized into lysosomes at the onset of lumenogenesis, but without CIDEA are prematurely degraded by cytosolic lipases. Loss of lipid storage or inactivation of lipophagy leads to the aberrant formation of multiple cavities within disorganised epithelial structures. Thus, our study reveals an unexpected role for LDs in orchestrating tissue remodelling and uncovers underappreciated facets of lipid metabolism in peri-implantation development.
Identifiants
pubmed: 35790717
doi: 10.1038/s41467-022-31323-2
pii: 10.1038/s41467-022-31323-2
pmc: PMC9256688
doi:
Substances chimiques
Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3861Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P005179/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/H020233/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P005209/2
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 103788/Z/14/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_1605/4
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_EX_MR/S015930/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/D52657X/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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