Scale-out production of extracellular vesicles derived from natural killer cells via mechanical stimulation in a seesaw-motion bioreactor for cancer therapy.

Bioreactors Extracellular Vesicles / metabolism Humans Killer Cells, Natural Melanoma / metabolism
bioreactor cancer therapy extracellular vesicles (EVs) mechanical stimulation natural killer cells (NKs)

Journal

Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964

Informations de publication

Date de publication:
05 08 2022
Historique:
received: 22 12 2021
accepted: 06 07 2022
pubmed: 7 7 2022
medline: 9 8 2022
entrez: 6 7 2022
Statut: epublish

Résumé

Extracellular vesicles (EVs) derived from immune cells have shown great anti-cancer therapeutic potential. However, inefficiency in EV generation has considerably impeded the development of EV-based basic research and clinical translation. Here, we developed a seesaw-motion bioreactor (SMB) system by leveraging mechanical stimuli such as shear stress and turbulence for generating EVs with high quality and quantity from natural killer (NK) cells. Compared to EV production in traditional static culture (229 ± 74 particles per cell per day), SMB produced NK-92MI-derived EVs at a higher rate of 438 ± 50 particles per cell per day and yielded a total number of 2 × 10

Identifiants

DOI: 10.1088/1758-5090/ac7eeb PMID: 35793612
pubmed: 35793612
doi: 10.1088/1758-5090/ac7eeb
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Creative Commons Attribution license.

Auteurs

Jianguo Wu (J)

Department of Respiratory and Critical Care Medicine, Provincial Clinical Research Center for Respiratory Diseases, West China Hospital, Sichuan University, Chengdu 610065, People's Republic of China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, People's Republic of China.
Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Di Wu (D)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Guohua Wu (G)

Endocrine and Metabolic Disease Center, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Medical Key Laboratory of Hereditary Rare Diseases of Henan; Luoyang sub-center of National Clinical Research Center for Metabolic Diseases, Luoyang 471003, People's Republic of China.

Ho-Pan Bei (HP)

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region of China 999077, People's Republic of China.

Zihan Li (Z)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Han Xu (H)

Department of Building Environment and Energy Engineering, Xi'an Jiaotong University, Xian, Shanxi Province 710049, People's Republic of China.

Yimin Wang (Y)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Dan Wu (D)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Hui Liu (H)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Shengyu Shi (S)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Chao Zhao (C)

Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute and Department of Clinical Neurosciences, University of Cambridge, Cambridge CB2 0AH, United Kingdom.

Yibing Xu (Y)

Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.

Yong He (Y)

State Key Laboratory of Fluid Power and Mechatronic Systems, Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical Engineering, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.
ORCID: 0000-0002-9099-0831

Jun Li (J)

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310003, People's Republic of China.

Changyong Wang (C)

Department of Neural Engineering and Biological Interdisciplinary Studies, Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Beijing 100850, People's Republic of China.

Xin Zhao (X)

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region of China 999077, People's Republic of China.
ORCID: 0000-0002-9521-7768

Shuqi Wang (S)

Department of Respiratory and Critical Care Medicine, Provincial Clinical Research Center for Respiratory Diseases, West China Hospital, Sichuan University, Chengdu 610065, People's Republic of China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, People's Republic of China.
Institute for Translational Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310029, People's Republic of China.
Institute for Advanced Study, Chengdu University, Chengdu 610106, People's Republic of China.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Bioréacteurs › Scale-out production of extracellular vesicles...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Vésicules extracellulaires › Scale-out production of extracellular vesicles...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Scale-out production of extracellular vesicles...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Cellules tueuses naturelles › Scale-out production of extracellular vesicles...
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Naevus et mélanomes › Mélanome › Scale-out production of extracellular vesicles...