Intersectional mapping of multi-transmitter neurons and other cell types in the brain.

Animals Habenula / metabolism Mice Mice, Transgenic Neurons / metabolism Vesicular Glutamate Transport Proteins / metabolism
CP: Neuroscience brain mapping intersectional strategy multi-transmitter neurons the lateral habenula

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
05 07 2022
Historique:
received: 13 09 2021
revised: 04 04 2022
accepted: 13 06 2022
entrez: 6 7 2022
pubmed: 7 7 2022
medline: 9 7 2022
Statut: ppublish

Résumé

Recent developments in intersectional strategies have greatly advanced our ability to precisely target brain cell types based on unique co-expression patterns. To accelerate the application of intersectional genetics, we perform a brain-wide characterization of 13 Flp and tTA mouse driver lines and selected seven for further analysis based on expression of vesicular neurotransmitter transporters. Using selective Cre driver lines, we created more than 10 Cre/tTA combinational lines for cell type targeting and circuit analysis. We then used VGLUT-Cre/VGAT-Flp combinational lines to identify and map 30 brain regions containing neurons that co-express vesicular glutamate and gamma-aminobutyric acid (GABA) transporters, followed by tracing their projections with intersectional viral vectors. Focusing on the lateral habenula (LHb) as a target, we identified glutamatergic, GABAergic, or co-glutamatergic/GABAergic innervations from ∼40 brain regions. These data provide an important resource for the future application of intersectional strategies and expand our understanding of the neuronal subtypes in the brain.

Identifiants

DOI: 10.1016/j.celrep.2022.111036 PMID: 35793636 PMC: PMC9290751
pubmed: 35793636
pii: S2211-1247(22)00830-0
doi: 10.1016/j.celrep.2022.111036
pmc: PMC9290751
mid: NIHMS1821577
pii:
doi:

Substances chimiques

Vesicular Glutamate Transport Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

111036

Subventions

Organisme : NEI NIH HHS
ID : R01 EY018204
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY030169
Pays : United States
Organisme : NEI NIH HHS
ID : F31 EY031985
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH099114
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH085500
Pays : United States
Organisme : NEI NIH HHS
ID : T32 EY025202
Pays : United States
Organisme : NIMH NIH HHS
ID : U19 MH114830
Pays : United States

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Jian Xu (J)

Departments of Ophthalmology and Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Andrew Jo (A)

Departments of Ophthalmology and Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Raina P DeVries (RP)

Department of Organismal Biology and Anatomy, The University of Chicago, Chicago, IL 60637, USA.

Sercan Deniz (S)

Departments of Ophthalmology and Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Suraj Cherian (S)

Departments of Ophthalmology and Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Idris Sunmola (I)

Departments of Ophthalmology and Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Xingqi Song (X)

School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

John J Marshall (JJ)

Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Katherine A Gruner (KA)

Mouse Histology and Phenotyping Laboratory, Northwestern University, Chicago, IL 60611, USA.

Tanya L Daigle (TL)

Allen Institute for Brain Science, Seattle, WA 98109, USA.

Anis Contractor (A)

Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Talia N Lerner (TN)

Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Hongkui Zeng (H)

Allen Institute for Brain Science, Seattle, WA 98109, USA.

Yongling Zhu (Y)

Departments of Ophthalmology and Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: yongling-zhu@northwestern.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Intersectional mapping of multi-transmitter...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Prosencéphale › Diencéphale › Épithalamus › Habénula › Intersectional mapping of multi-transmitter...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Intersectional mapping of multi-transmitter...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Intersectional mapping of multi-transmitter...
questionsmedicales.fr › Cellules › Neurones › Intersectional mapping of multi-transmitter...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs de neurotransmetteurs › Transporteurs vésiculaires des neurotransmetteurs › Transporteurs vésiculaires du glutamate › Intersectional mapping of multi-transmitter...