People with HIV have higher percentages of circulating CCR5+ CD8+ T cells and lower percentages of CCR5+ regulatory T cells.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
06 07 2022
Historique:
received: 18 01 2022
accepted: 27 06 2022
entrez: 6 7 2022
pubmed: 7 7 2022
medline: 9 7 2022
Statut: epublish

Résumé

CCR5 is the main HIV co-receptor. We aimed to (1) compare CCR5 expression on immune cells between people living with HIV (PLHIV) using combination antiretroviral therapy (cART) and HIV-uninfected controls, (2) relate CCR5 expression to viral reservoir size and (3) assess determinants of CCR5 expression. This cross-sectional study included 209 PLHIV and 323 controls. Percentages of CCR5+ cells (%) and CCR5 mean fluorescence intensity assessed by flow cytometry in monocytes and lymphocyte subsets were correlated to host factors, HIV-1 cell-associated (CA)-RNA and CA-DNA, plasma inflammation markers and metabolites. Metabolic pathways were identified. PLHIV displayed higher percentages of CCR5+ monocytes and several CD8+ T cell subsets, but lower percentages of CCR5+ naive CD4+ T cells and regulatory T cells (Tregs). HIV-1 CA-DNA and CA-RNA correlated positively with percentages of CCR5+ lymphocytes. Metabolome analysis revealed three pathways involved in energy metabolism associated with percentage of CCR5+ CD8+ T cells in PLHIV. Our results indicate that CCR5 is differently expressed on various circulating immune cells in PLHIV. Hence, cell-trafficking of CD8+ T cells and Tregs may be altered in PLHIV. Associations between energy pathways and percentage of CCR5+ CD8+ T cells in PLHIV suggest higher energy demand of these cells in PLHIV.

Identifiants

pubmed: 35794176
doi: 10.1038/s41598-022-15646-0
pii: 10.1038/s41598-022-15646-0
pmc: PMC9259737
doi:

Substances chimiques

CCR5 protein, human 0
Receptors, CCR5 0
Receptors, HIV 0
RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11425

Informations de copyright

© 2022. The Author(s).

Références

AIDS Res Ther. 2013 May 09;10(1):11
pubmed: 23659629
J Immunol. 2009 Jan 1;182(1):171-82
pubmed: 19109148
Cell Mol Immunol. 2020 Dec;17(12):1257-1265
pubmed: 32210395
Thromb Haemost. 2017 Jun 2;117(6):1141-1149
pubmed: 28276569
Virol J. 2006 Dec 22;3:104
pubmed: 17187670
J Exp Med. 1997 May 5;185(9):1681-91
pubmed: 9151905
J Virol. 1998 Jan;72(1):830-6
pubmed: 9420295
Annu Rev Immunol. 2004;22:745-63
pubmed: 15032595
Cell Rep. 2019 Sep 17;28(12):3011-3021.e4
pubmed: 31533027
Front Immunol. 2019 Nov 28;10:2795
pubmed: 31849973
J Virol. 1998 Jun;72(6):4962-9
pubmed: 9573265
Open Forum Infect Dis. 2019 Mar 07;6(4):ofz112
pubmed: 30968058
Front Pharmacol. 2020 Mar 03;11:146
pubmed: 32194402
PLoS One. 2007 May 23;2(5):e470
pubmed: 17520029
Nat Rev Immunol. 2020 Aug;20(8):471-482
pubmed: 32051540
Nature. 1999 Oct 14;401(6754):708-12
pubmed: 10537110
J Infect Dis. 2007 Aug 1;196(3):371-6
pubmed: 17597451
Ann Intern Med. 2001 Jan 2;134(1):81-2
pubmed: 11187428
J Infect Dis. 2015 Nov 1;212(9):1397-401
pubmed: 25895986
Proc Natl Acad Sci U S A. 2015 Aug 25;112(34):E4762-71
pubmed: 26307764
FASEB J. 2019 Aug;33(8):8905-8912
pubmed: 31034775
Sci Rep. 2021 Mar 18;11(1):6246
pubmed: 33739024
Mediators Inflamm. 2003 Feb;12(1):29-35
pubmed: 12745546
J Immunol. 1998 Sep 15;161(6):3195-201
pubmed: 9743388
Arterioscler Thromb Vasc Biol. 2015 Jan;35(1):40-9
pubmed: 25301842
Nat Immunol. 2018 Jul;19(7):776-786
pubmed: 29784908
JCI Insight. 2021 Apr 8;6(7):
pubmed: 33630761
AIDS. 2017 Nov 28;31(18):2443-2454
pubmed: 28926402
Nucleic Acids Res. 2017 Jan 4;45(D1):D353-D361
pubmed: 27899662
Biol Psychiatry. 2016 Jul 1;80(1):33-9
pubmed: 26725193
Int J Immunogenet. 2020 Jun;47(3):261-285
pubmed: 32212259
AIDS. 2020 May 1;34(6):833-848
pubmed: 32044843
Immunity. 2019 Nov 19;51(5):856-870.e5
pubmed: 31747582
Clin Immunol. 1999 Jun;91(3):254-62
pubmed: 10370370
Metabolites. 2019 Sep 30;9(10):
pubmed: 31574898
J Virol. 2007 Dec;81(24):13865-75
pubmed: 17898067
PLoS Comput Biol. 2013;9(7):e1003123
pubmed: 23861661
J Infect Dis. 2014 Dec 1;210(11):1838-43
pubmed: 24935955
Cell. 1996 Aug 9;86(3):367-77
pubmed: 8756719
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
J Immunol Methods. 2003 Jul;278(1-2):145-55
pubmed: 12957403
BMC Infect Dis. 2014 Dec 13;14:683
pubmed: 25495598
Blood. 2000 Oct 15;96(8):2649-54
pubmed: 11023494
AIDS. 2013 Apr 24;27(7):1059-69
pubmed: 23299175
Basic Res Cardiol. 2016 Nov;111(6):71
pubmed: 27783202
PLoS Pathog. 2020 Aug 13;16(8):e1008679
pubmed: 32790802
Nucleic Acids Res. 2018 Jul 2;46(W1):W486-W494
pubmed: 29762782
J Leukoc Biol. 2002 Oct;72(4):735-42
pubmed: 12377943
Int J Mol Sci. 2020 Feb 21;21(4):
pubmed: 32098198
AIDS. 2001 Apr 13;15(6):735-46
pubmed: 11371688
Cell Rep. 2016 Nov 22;17(9):2474-2487
pubmed: 27818087
Anal Chem. 2011 Sep 15;83(18):7074-80
pubmed: 21830798
J Immunol. 2003 Jan 15;170(2):895-904
pubmed: 12517955
J Immunol. 1999 Oct 15;163(8):4597-603
pubmed: 10510404
Respir Res. 2017 Sep 8;18(1):170
pubmed: 28886713
mBio. 2021 Mar 9;12(2):
pubmed: 33688006
J Infect Dis. 2016 Mar 1;213(5):766-70
pubmed: 26512140
Cancers (Basel). 2020 Jul 02;12(7):
pubmed: 32630699
Biometrics. 2020 Dec;76(4):1262-1272
pubmed: 31883270
J Infect Dis. 2000 Mar;181(3):927-32
pubmed: 10720514
Virus Res. 2020 Sep;286:198040
pubmed: 32479976
Front Immunol. 2019 Aug 27;10:1965
pubmed: 31507593
J Acquir Immune Defic Syndr. 2002 Feb 1;29(2):122-31
pubmed: 11832680
J Mol Biol. 2018 Aug 17;430(17):2557-2589
pubmed: 29932942
Immunity. 2009 Jun 19;30(6):899-911
pubmed: 19464196

Auteurs

Louise E van Eekeren (LE)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands. Louise.vanEekeren@radboudumc.nl.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands. Louise.vanEekeren@radboudumc.nl.
Radboud Institute of Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands. Louise.vanEekeren@radboudumc.nl.

Vasiliki Matzaraki (V)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.

Zhenhua Zhang (Z)

Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Lisa van de Wijer (L)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute of Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Marc J T Blaauw (MJT)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute of Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Marien I de Jonge (MI)

Department of Laboratory Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Linos Vandekerckhove (L)

HIV Cure Research Center, Department of Internal Medicine, and Pediatrics, Ghent University & Ghent University Hospital, Ghent, Belgium.

Wim Trypsteen (W)

HIV Cure Research Center, Department of Internal Medicine, and Pediatrics, Ghent University & Ghent University Hospital, Ghent, Belgium.

Leo A B Joosten (LAB)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Mihai G Netea (MG)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Quirijn de Mast (Q)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute of Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Hans J P M Koenen (HJPM)

Department of Laboratory Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.

Yang Li (Y)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
Department of Computational Biology for Individualised Medicine, Centre for Individualised Infection Medicine (CiiM) & TWINCORE, Joint Ventures Between the Helmholtz-Centre for Infection Research (HZI) and the Hannover Medical School (MHH), Hannover, Germany.

André J A M van der Ven (AJAM)

Department of General Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Radboud Institute of Health Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH