3D Bioprinted Spheroidal Droplets for Engineering the Heterocellular Coupling between Cardiomyocytes and Cardiac Fibroblasts.
Journal
Cyborg and bionic systems (Washington, D.C.)
ISSN: 2692-7632
Titre abrégé: Cyborg Bionic Syst
Pays: United States
ID NLM: 9918400086506676
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
7
7
2022
pubmed:
1
1
2021
medline:
1
1
2021
Statut:
ppublish
Résumé
Since conventional human cardiac two-dimensional (2D) cell culture and multilayered three-dimensional (3D) models fail in recapitulating cellular complexity and possess inferior translational capacity, we designed and developed a high-throughput scalable 3D bioprinted cardiac spheroidal droplet-organoid model with cardiomyocytes and cardiac fibroblasts that can be used for drug screening or regenerative engineering applications. This study helped establish the parameters for bioprinting and cross-linking a gelatin-alginate-based bioink into 3D spheroidal droplets. A flattened disk-like structure developed in prior studies from our laboratory was used as a control. The microstructural and mechanical stability of the 3D spheroidal droplets was assessed and was found to be ideal for a cardiac scaffold. Adult human cardiac fibroblasts and AC16 cardiomyocytes were mixed in the bioink and bioprinted. Live-dead assay and flow cytometry analysis revealed robust biocompatibility of the 3D spheroidal droplets that supported the growth and proliferation of the cardiac cells in the long-term cultures. Moreover, the heterocellular gap junctional coupling between the cardiomyocytes and cardiac fibroblasts further validated the 3D cardiac spheroidal droplet model.
Identifiants
pubmed: 35795473
doi: 10.34133/2021/9864212
pmc: PMC9254634
mid: NIHMS1814163
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NHLBI NIH HHS
ID : SC1 HL154511
Pays : United States
Déclaration de conflit d'intérêts
Conflicts of Interest The authors declare no conflicts of interest regarding the publication of this article.
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