Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies.
Caspase 9
gene editing
induced pluripotent stem cells
loss of heterozygosity
silencing
suicide gene
Journal
Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857
Informations de publication
Date de publication:
08 Sep 2022
08 Sep 2022
Historique:
received:
17
12
2021
accepted:
25
05
2022
entrez:
7
7
2022
pubmed:
8
7
2022
medline:
8
7
2022
Statut:
epublish
Résumé
Drug-inducible suicide systems may help to minimize risks of human induced pluripotent stem cell (hiPSC) therapies. Recent research challenged the usefulness of such systems since rare drug-resistant subclones were observed. We have introduced a drug-inducible Caspase 9 suicide system (iCASP9) into the AAVS1 safe-harbor locus of hiPSCs. In these cells, apoptosis could be efficiently induced
Identifiants
pubmed: 35795779
doi: 10.1016/j.omtm.2022.05.011
pii: S2329-0501(22)00076-6
pmc: PMC9234009
doi:
Types de publication
Journal Article
Langues
eng
Pagination
84-94Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors have no commercial, proprietary, or financial interest in the products or companies described in this article.
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