Preliminary investigation of gene expression levels of PAPP-A, STC-2, and HIF-1α in SARS-Cov-2 infected patients.

COVID-19 / genetics Gene Expression Glycoproteins / genetics Humans Hypoxia-Inducible Factor 1, alpha Subunit / genetics Intercellular Signaling Peptides and Proteins / genetics Pregnancy-Associated Plasma Protein-A / genetics RNA, Messenger / genetics SARS-CoV-2
COVID-19 HIF-1α PAPP-A SARS-CoV-2 STC-2

Journal

Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234

Informations de publication

Date de publication:
Sep 2022
Historique:
received: 31 03 2022
accepted: 14 06 2022
pubmed: 8 7 2022
medline: 14 9 2022
entrez: 7 7 2022
Statut: ppublish

Résumé

Coronavirus-19 is still considered a pandemic that influences the world. Other molecular alterations should be clearer besides the increasing cytokine storm and pro-inflammatory molecules. Hypoxic conditions that induce HIF-1α lead to stimulate gene expression of STC-2 that targets PAPP-A expression. This study aimed to determine gene expression levels of PAPP-A, STC-2, and HIF-1α in COVID-19 infection. We also aimed to reveal the relationship of these genes with laboratory and clinical data of COVID-19 patients. We extracted RNA from peripheral blood samples of COVID-19(+) and COVID-19(-) individuals. The real-time PCR method was used to measure mRNA expression of PAPP-A, STC-2, and HIF-1α. Gene expression analysis was evaluated by the 2 This is the first study that investigates the relation of PAPP-A, STC-2, and HIF-1α gene expression in patients with COVID-19 infection. Besides the routine laboratory findings, PAPP-A, STC-2, and HIF-1α mRNA expressions may be considered to patients' prognosis as a sign of increased cytokines and pro-inflammatory molecules.

Sections du résumé

BACKGROUND BACKGROUND
Coronavirus-19 is still considered a pandemic that influences the world. Other molecular alterations should be clearer besides the increasing cytokine storm and pro-inflammatory molecules. Hypoxic conditions that induce HIF-1α lead to stimulate gene expression of STC-2 that targets PAPP-A expression. This study aimed to determine gene expression levels of PAPP-A, STC-2, and HIF-1α in COVID-19 infection. We also aimed to reveal the relationship of these genes with laboratory and clinical data of COVID-19 patients.
MATERIALS AND RESULTS RESULTS
We extracted RNA from peripheral blood samples of COVID-19(+) and COVID-19(-) individuals. The real-time PCR method was used to measure mRNA expression of PAPP-A, STC-2, and HIF-1α. Gene expression analysis was evaluated by the 2
CONCLUSION CONCLUSIONS
This is the first study that investigates the relation of PAPP-A, STC-2, and HIF-1α gene expression in patients with COVID-19 infection. Besides the routine laboratory findings, PAPP-A, STC-2, and HIF-1α mRNA expressions may be considered to patients' prognosis as a sign of increased cytokines and pro-inflammatory molecules.

Identifiants

DOI: 10.1007/s11033-022-07710-9 PMID: 35796937 PMC: PMC9261127
pubmed: 35796937
doi: 10.1007/s11033-022-07710-9
pii: 10.1007/s11033-022-07710-9
pmc: PMC9261127
doi:

Substances chimiques

Glycoproteins 0
HIF1A protein, human 0
Hypoxia-Inducible Factor 1, alpha Subunit 0
Intercellular Signaling Peptides and Proteins 0
RNA, Messenger 0
STC2 protein, human 0
Pregnancy-Associated Plasma Protein-A EC 3.4.24.-
PAPPA protein, human EC 3.4.24.79

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

8693-8699

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer Nature B.V.

Références

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Auteurs

Sevgi Karabulut Uzunçakmak (S)

Health Services Vocational School, Bayburt University, 69000, Bayburt, Turkey. skarabulut@bayburt.edu.tr.
ORCID: 0000-0001-9714-0349

Muhammet Emin Naldan (ME)

Department of Anesthesia, Bilecik Seyh Edibali University, 11230, Bilecik, Turkey.

Ebubekir Dirican (E)

Health Services Vocational School, Bayburt University, 69000, Bayburt, Turkey.

Ferhan Kerget (F)

Department of Infectious and Clinical Microbiology Diseases, Erzurum Regional Education and Research Hospital, 25240, Erzurum, Turkey.

Zekai Halıcı (Z)

Department of Pharmacology, Ataturk University, 25240, Erzurum, Turkey.
Clinical Research, Development and Design Application and Research Center, Ataturk University, 25240, Erzurum, Turkey.

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Classifications MeSH

questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Preliminary investigation of gene expression...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Preliminary investigation of gene expression...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Glycoprotéines › Preliminary investigation of gene expression...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Preliminary investigation of gene expression...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique hélice-boucle-hélice › Facteur-1 induit par l'hypoxie › Sous-unité alpha du facteur-1 induit par l'hypoxie › Preliminary investigation of gene expression...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Preliminary investigation of gene expression...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de la grossesse › Protéine A plasmatique associée à la grossesse › Preliminary investigation of gene expression...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Preliminary investigation of gene expression...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Preliminary investigation of gene expression...