FRAGLER: A Fragment Recycler Application Enabling Rapid and Scalable Modular DNA Assembly.


Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
15 07 2022
Historique:
pubmed: 8 7 2022
medline: 19 7 2022
entrez: 7 7 2022
Statut: ppublish

Résumé

Rapid and flexible plasmid construct generation at scale is one of the most limiting first steps in drug discovery projects. These hurdles can partly be overcome by adopting modular DNA design principles, automated sequence fragmentation, and plasmid assembly. To this end we have designed a robust, multimodule

Identifiants

pubmed: 35797032
doi: 10.1021/acssynbio.2c00106
pmc: PMC9295698
doi:

Substances chimiques

RNA, Viral 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2229-2237

Références

ACS Synth Biol. 2021 Dec 17;10(12):3422-3434
pubmed: 34767345
Appl Microbiol Biotechnol. 2011 Aug;91(3):731-40
pubmed: 21556920
Sci Rep. 2019 Jul 12;9(1):10131
pubmed: 31300661
FEBS Lett. 1997 Jul 28;412(2):388-96
pubmed: 9256258
Biologics. 2021 Apr 14;15:95-105
pubmed: 33883875
PLoS One. 2017 May 17;12(5):e0176314
pubmed: 28520717
PLoS One. 2011;6(7):e21622
pubmed: 21750718
ACS Synth Biol. 2018 Nov 16;7(11):2665-2674
pubmed: 30335370
J Mol Biol. 2004 Dec 3;344(4):1051-69
pubmed: 15544812
J Biol Chem. 2015 Mar 20;290(12):7436-42
pubmed: 25635058
Biochem Soc Trans. 2016 Apr 15;44(2):412-8
pubmed: 27068948
PLoS One. 2011 Feb 18;6(2):e16765
pubmed: 21364738
PLoS One. 2020 Sep 2;15(9):e0238592
pubmed: 32877448
ACS Synth Biol. 2015 Sep 18;4(9):975-86
pubmed: 25871405
ACS Synth Biol. 2017 Jun 16;6(6):1016-1025
pubmed: 28252957
ACS Synth Biol. 2016 Oct 21;5(10):1059-1069
pubmed: 27096716
Cancer Res. 2009 Dec 15;69(24):9385-94
pubmed: 19996290
ACS Synth Biol. 2019 Nov 15;8(11):2593-2606
pubmed: 31686495
PLoS One. 2008;3(11):e3647
pubmed: 18985154
Trends Immunol. 2015 Aug;36(8):494-502
pubmed: 26169254
ACS Synth Biol. 2017 Jul 21;6(7):1380-1392
pubmed: 28418644
JCI Insight. 2018 Jan 11;3(1):
pubmed: 29321369
ACS Synth Biol. 2022 Feb 18;11(2):579-586
pubmed: 35050610

Auteurs

David Öling (D)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Olivier Lan-Chow-Wing (O)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.

Andrea Martella (A)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.

Samuel Gilberto (S)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.

Jordi Chi (J)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Emily Cooper (E)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.

Tora Edström (T)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Bo Peng (B)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Dean Sumner (D)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Fredrik Karlsson (F)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Petr Volkov (P)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Carl I Webster (CI)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.

Robert Roth (R)

Discovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

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Classifications MeSH