EWAS of post-COVID-19 patients shows methylation differences in the immune-response associated gene, IFI44L, three months after COVID-19 infection.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
07 07 2022
Historique:
received: 22 10 2021
accepted: 23 06 2022
entrez: 7 7 2022
pubmed: 8 7 2022
medline: 12 7 2022
Statut: epublish

Résumé

Although substantial progress has been made in managing COVID-19, it is still difficult to predict a patient's prognosis. We explored the epigenetic signatures of COVID-19 in peripheral blood using data from an ongoing prospective observational study of COVID-19 called the Norwegian Corona Cohort Study. A series of EWASs were performed to compare the DNA methylation profiles between COVID-19 cases and controls three months post-infection. We also investigated differences associated with severity and long-COVID. Three CpGs-cg22399236, cg03607951, and cg09829636-were significantly hypomethylated (FDR < 0.05) in COVID-19 positive individuals. cg03607951 is located in IFI44L which is involved in innate response to viral infection and several systemic autoimmune diseases. cg09829636 is located in ANKRD9, a gene implicated in a wide variety of cellular processes, including the degradation of IMPDH2. The link between ANKRD9 and IMPDH2 is striking given that IMPDHs are considered therapeutic targets for COVID-19. Furthermore, gene ontology analyses revealed pathways involved in response to viruses. The lack of significant differences associated with severity and long-COVID may be real or reflect limitations in sample size. Our findings support the involvement of interferon responsive genes in the pathophysiology of COVID-19 and indicate a possible link to systemic autoimmune diseases.

Identifiants

pubmed: 35798818
doi: 10.1038/s41598-022-15467-1
pii: 10.1038/s41598-022-15467-1
pmc: PMC9261254
doi:

Types de publication

Journal Article Observational Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11478

Informations de copyright

© 2022. The Author(s).

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Auteurs

Yunsung Lee (Y)

Centre for Fertility and Health, Norwegian Institute of Public Health, Skøyen, P.O. box 222, 0213, Oslo, Norway.

Espen Riskedal (E)

Age Labs AS, Gaustadalléen 23A, 0373, Oslo, Norway.

Karl Trygve Kalleberg (KT)

Age Labs AS, Gaustadalléen 23A, 0373, Oslo, Norway.

Mette Istre (M)

Department of Microbiology, Oslo University Hospital Rikshospitalet, 0372, Oslo, Norway.

Andreas Lind (A)

Department of Microbiology, Oslo University Hospital Ullevaal, 0372, Oslo, Norway.

Fridtjof Lund-Johansen (F)

Department of Immunology, Oslo University Hospital Rikshospitalet, 0372, Oslo, Norway.

Olaug Reiakvam (O)

Department of Microbiology, Oslo University Hospital Rikshospitalet, 0372, Oslo, Norway.

Arne V L Søraas (AVL)

Department of Microbiology, Oslo University Hospital Rikshospitalet, 0372, Oslo, Norway.

Jennifer R Harris (JR)

Centre for Fertility and Health, Norwegian Institute of Public Health, Skøyen, P.O. box 222, 0213, Oslo, Norway.

John Arne Dahl (JA)

Department of Microbiology, Oslo University Hospital Rikshospitalet, 0372, Oslo, Norway.

Cathrine L Hadley (CL)

Age Labs AS, Gaustadalléen 23A, 0373, Oslo, Norway. cathrine@agelabs.com.

Astanand Jugessur (A)

Centre for Fertility and Health, Norwegian Institute of Public Health, Skøyen, P.O. box 222, 0213, Oslo, Norway.
Department of Global Public Health and Primary Care, University of Bergen, P.O. box 7804, 5020, Bergen, Norway.

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Classifications MeSH