Human liver organoids for disease modeling of fibrolamellar carcinoma.
fibrolamellar
liver cancer
pediatric cancer
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
09 08 2022
09 08 2022
Historique:
received:
15
01
2022
revised:
04
06
2022
accepted:
07
06
2022
pubmed:
9
7
2022
medline:
13
8
2022
entrez:
8
7
2022
Statut:
ppublish
Résumé
Fibrolamellar carcinoma (FLC) is a rare, often lethal, liver cancer affecting adolescents and young adults, for which there are no approved therapeutics. The development of therapeutics is hampered by a lack of in vitro models. Organoids have shown utility as a model system for studying many diseases. In this study, tumor tissue and the adjacent non-tumor liver were obtained at the time of surgery. The tissue was dissociated and grown as organoids. We developed 21 patient-derived organoid lines: 12 from metastases, three from the liver tumor and six from adjacent non-tumor liver. These patient-derived FLC organoids recapitulate the histologic morphology, immunohistochemistry, and transcriptome of the patient tumor. Patient-derived FLC organoids were used in a preliminary high-throughput drug screen to show proof of concept for the identification of therapeutics. This model system has the potential to improve our understanding of this rare cancer and holds significant promise for drug testing and development.
Identifiants
pubmed: 35803261
pii: S2213-6711(22)00319-8
doi: 10.1016/j.stemcr.2022.06.003
pmc: PMC9391427
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1874-1888Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR001866
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R56 CA207929
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA210964
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA243126
Pays : United States
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
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