In Vitro and Ectopic In Vivo Studies toward the Utilization of
arthroscopy
autologous chondrocyte implantation
cartilage regeneration
hydrogel
nasal chondrocytes
platelet lysate
polyethylene glycol
single-stage
tissue engineering
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Jun 2022
21 Jun 2022
Historique:
received:
21
05
2022
revised:
15
06
2022
accepted:
19
06
2022
entrez:
9
7
2022
pubmed:
10
7
2022
medline:
14
7
2022
Statut:
epublish
Résumé
Nasal chondrocytes (NCs) have a higher and more reproducible chondrogenic capacity than articular chondrocytes, and the engineered cartilage tissue they generate in vitro has been demonstrated to be safe in clinical applications. Here, we aimed at determining the feasibility for a single-stage application of NCs for cartilage regeneration under minimally invasive settings. In particular, we assessed whether NCs isolated using a short collagenase digestion protocol retain their potential to proliferate and chondro-differentiate within an injectable, swiftly cross-linked and matrix-metalloproteinase (MMP)-degradable polyethylene glycol (PEG) gel enriched with human platelet lysate (hPL). NC-hPL-PEG gels were additionally tested for their capacity to generate cartilage tissue in vivo and to integrate into cartilage/bone compartments of human osteochondral plugs upon ectopic subcutaneous implantation into nude mice. NCs isolated with a
Identifiants
pubmed: 35805907
pii: ijms23136900
doi: 10.3390/ijms23136900
pmc: PMC9267018
pii:
doi:
Substances chimiques
Hydrogels
0
Polyethylene Glycols
3WJQ0SDW1A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
J Cell Biochem. 2010 May;110(1):260-71
pubmed: 20213765
Tissue Eng. 2004 Mar-Apr;10(3-4):515-22
pubmed: 15165468
Tissue Eng Part A. 2013 Jun;19(11-12):1476-88
pubmed: 23360471
Biomaterials. 2010 Oct;31(30):7836-45
pubmed: 20667588
J Tissue Eng Regen Med. 2008 Dec;2(8):472-81
pubmed: 18932128
Am J Sports Med. 2019 Sep;47(11):2584-2588
pubmed: 31336053
Connect Tissue Res. 2003;44(3-4):173-80
pubmed: 14504038
Plast Reconstr Surg. 2002 Sep 15;110(4):1073-9
pubmed: 12198420
N Engl J Med. 1994 Oct 6;331(14):889-95
pubmed: 8078550
Adv Healthc Mater. 2020 Apr;9(7):e1901669
pubmed: 32129003
Biomacromolecules. 2007 Oct;8(10):3000-7
pubmed: 17883273
Adv Healthc Mater. 2015 Apr 2;4(5):702-13
pubmed: 25607633
Acta Biomater. 2019 Jan 1;83:233-244
pubmed: 30366137
Nat Biotechnol. 2005 Jan;23(1):47-55
pubmed: 15637621
Arch Facial Plast Surg. 2003 Jan-Feb;5(1):53-8
pubmed: 12533140
Biomed Res Int. 2017;2017:2395138
pubmed: 28337445
J Cell Mol Med. 2015 Jun;19(6):1390-9
pubmed: 25689393
J Tissue Eng Regen Med. 2015 Jul;9(7):808-18
pubmed: 23303715
Nat Rev Mater. 2016;1:
pubmed: 29214058
Lasers Surg Med. 2021 Mar;53(3):377-389
pubmed: 32614077
Front Bioeng Biotechnol. 2020 Apr 07;8:283
pubmed: 32318561
Int J Mol Sci. 2021 Apr 08;22(8):
pubmed: 33917689
Lancet. 2016 Oct 22;388(10055):1985-1994
pubmed: 27789021
Eur Cell Mater. 2008 Feb 05;15:1-10
pubmed: 18247273
Osteoarthritis Cartilage. 2013 Jul;21(7):950-6
pubmed: 23583464
Laryngoscope. 2006 Oct;116(10):1730-4
pubmed: 17003728
JBJS Rev. 2021 Jan 19;9(1):e20.00090
pubmed: 33512974
Stem Cells. 2017 Jan;35(1):256-264
pubmed: 27507787
Cells. 2019 Jul 15;8(7):
pubmed: 31311198
EMBO Rep. 2018 Aug;19(8):
pubmed: 29967223
Health Technol Assess. 2017 Feb;21(6):1-294
pubmed: 28244303
Laryngoscope. 2009 Sep;119(9):1770-5
pubmed: 19554638
IEEE Trans Ultrason Ferroelectr Freq Control. 2019 Sep;66(9):1435-1443
pubmed: 31226071
Arch Biochem Biophys. 2002 Jul 1;403(1):132-40
pubmed: 12061810
Eur Cell Mater. 2018 May 1;35:255-267
pubmed: 29714398
Front Bioeng Biotechnol. 2020 Jul 08;8:804
pubmed: 32733874
Acta Orthop Belg. 2010 Oct;76(5):669-74
pubmed: 21138224
Osteoarthritis Cartilage. 2004 Jun;12(6):476-84
pubmed: 15135144
Adv Healthc Mater. 2015 Mar 11;4(4):550-8
pubmed: 25358649
Biomaterials. 2007 Sep;28(26):3856-66
pubmed: 17568666
Cells. 2021 Sep 21;10(9):
pubmed: 34572145
Stem Cells. 2016 Dec;34(12):2956-2966
pubmed: 27538760
Matrix Biol. 2014 Oct;39:25-32
pubmed: 25172825
Tissue Eng Part C Methods. 2013 Oct;19(10):794-801
pubmed: 23448137
Stem Cell Res Ther. 2016 Jul 13;7(1):93
pubmed: 27411942
Am J Sports Med. 2020 May;48(6):1327-1337
pubmed: 32267734
Biomaterials. 2012 May;33(14):3651-61
pubmed: 22349290
Am J Sports Med. 2013 Sep;41(9):2158-66
pubmed: 23831891
J Surg Res. 2013 May 1;181(1):41-8
pubmed: 22819310
Am J Sports Med. 2018 May;46(6):1343-1351
pubmed: 29565642
Am J Sports Med. 2017 Oct;45(12):2751-2761
pubmed: 28745972
J Laryngol Otol. 2017 Jun;131(6):529-533
pubmed: 28316288
Tissue Eng Part C Methods. 2015 Apr;21(4):394-403
pubmed: 25226356
Matrix Biol. 2018 Oct;71-72:40-50
pubmed: 29800616
Biomaterials. 2018 Jul;171:219-229
pubmed: 29705655
Biomaterials. 2011 Feb;32(5):1301-10
pubmed: 21040970
Osteoarthritis Cartilage. 2014 Nov;22(11):1910-7
pubmed: 25151084
Am J Sports Med. 2010 Jun;38(6):1117-24
pubmed: 20181804
J Biomed Opt. 2014 Sep;19(9):98001
pubmed: 25200394
Cell Transplant. 2011;20(6):797-811
pubmed: 21092406
Sports Health. 2016 Mar-Apr;8(2):153-60
pubmed: 26502188
Tissue Eng. 2006 Aug;12(8):2141-9
pubmed: 16968155