Shear Stress Enhances the Paracrine-Mediated Immunoregulatory Function of Human Periodontal Ligament Stem Cells via the ERK Signalling Pathway.
IDO
T cells
TGF-β1
immunosuppression
mechanotransduction
periodontal ligament stem cells
shear stress
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 Jun 2022
27 Jun 2022
Historique:
received:
27
05
2022
revised:
16
06
2022
accepted:
23
06
2022
entrez:
9
7
2022
pubmed:
10
7
2022
medline:
14
7
2022
Statut:
epublish
Résumé
Relevant immunomodulatory effects have been proposed following allogeneic cell-based therapy with human periodontal ligament stem cells (hPDLSCs). This study aimed to examine the influence of shear stress on the immunosuppressive capacity of hPDLSCs. Cells were subjected to shear stress at different magnitudes (0.5, 5 and 10 dyn/cm2). The expression of immunosuppressive markers was evaluated in shear stress-induced hPDLSCs using qRT-PCR, western blot, enzyme activity and enzyme-linked immunosorbent assays. The effects of a shear stress-derived condition medium (SS-CM) on T cell proliferation were examined using a resazurin assay. Treg differentiation was investigated using qRT-PCR and flow cytometry analysis. Our results revealed that shear stress increased mRNA expression of
Identifiants
pubmed: 35806124
pii: ijms23137119
doi: 10.3390/ijms23137119
pmc: PMC9266779
pii:
doi:
Substances chimiques
Kynurenine
343-65-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the Graduate School, Chulalongkorn University to commemorate the 72nd Anniversary of his Majesty king Bhumibol Aduladej
ID : N/A
Organisme : Thailand Science Research and Innovation Fund, Chulalongkorn University
ID : CU-FRB65-_hea(10)_016_32_11
Organisme : Faculty Research Grant, Faculty of Dentistry, Chulalongkorn University
ID : DRF60019
Organisme : NSRF via the Program Management Unit for Human Resources & Institutional Development, Research, and Innovation
ID : B16F640118
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