Chlamydia psittaci plasmid-encoded CPSIT_P7 induces macrophage polarization to enhance the antibacterial response through TLR4-mediated MAPK and NF-κB pathways.

Animals Anti-Bacterial Agents / metabolism Chlamydophila psittaci / genetics Macrophages / metabolism Mice Mitogen-Activated Protein Kinases / metabolism NF-kappa B / genetics Plasmids / genetics Psittacosis / microbiology Toll-Like Receptor 4 / genetics
Antibacterial response Chlamydia psittaci Innate immune MAPK Macrophage polarization NF-κB

Journal

Biochimica et biophysica acta. Molecular cell research
ISSN: 1879-2596
Titre abrégé: Biochim Biophys Acta Mol Cell Res
Pays: Netherlands
ID NLM: 101731731

Informations de publication

Date de publication:
10 2022
Historique:
received: 28 03 2022
revised: 23 06 2022
accepted: 30 06 2022
pubmed: 10 7 2022
medline: 10 8 2022
entrez: 9 7 2022
Statut: ppublish

Résumé

Although the protective effects of Chlamydia psittaci plasmid-encoded protein CPSIT_P7 as vaccine antigens to against chlamydial infection have been confirmed in our previous study, the function and mechanism of CPSIT_P7 inducing innate immunity in the antibacterial response remain unknown. Here, we found that plasmid protein CPSIT_P7 could induce M1 macrophage polarization upregulating the genes of the surface molecule CD86, proinflammatory cytokines (TNF-α, IL-6, and IL-1β), and antibacterial effector NO synthase 2 (iNOS). During M1 macrophage polarization, macrophages acquire phagocytic and microbicidal competence, which promotes the host antibacterial response. As we observed that CPSIT_P7-induced M1 macrophages could partially reduce the infected mice pulmonary Chlamydia psittaci load. Furthermore, CPSIT_P7 induced M1 macrophage polarization through the TLR4-mediated MAPK and NF-κB pathways. Collectively, our results highlight the effect of CPSIT_P7 on macrophage polarization and provide new insights into new prevention and treatment strategies for chlamydial infection.

Identifiants

DOI: 10.1016/j.bbamcr.2022.119324 PMID: 35809864
pubmed: 35809864
pii: S0167-4889(22)00116-1
doi: 10.1016/j.bbamcr.2022.119324
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
NF-kappa B 0
Tlr4 protein, mouse 0
Toll-Like Receptor 4 0
Mitogen-Activated Protein Kinases EC 2.7.11.24

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

119324

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Siqin He (S)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China.

Chuan Wang (C)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China.

Yanru Huang (Y)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China.

Simin Lu (S)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China.

Weiwei Li (W)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China.

Nan Ding (N)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China.

Chaoqun Chen (C)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China. Electronic address: chaoqunch@163.com.

Yimou Wu (Y)

Institute of Pathogenic Biology, Hengyang Medical College, University of South China, China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, Hunan 421001, China. Electronic address: yimouwu@sina.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Chlamydiales › Chlamydiaceae › Chlamydophila › Chlamydophila psittaci › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Mitogen-Activated Protein Kinases › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteur de transcription NF-kappa B › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Chlamydiaceae › Infections à Chlamydophila › Psittacose › Chlamydia psittaci plasmid-encoded CPSIT_P7...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs de reconnaissance de motifs moléculaires › Récepteurs de type Toll › Récepteur de type Toll-4 › Chlamydia psittaci plasmid-encoded CPSIT_P7...